Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using Phospho-HSP27 (Ser82) Antibody (upper) or control HSP27 antibody (lower). HeLa cells were incubated at 42°C for 0–2 hours as indicated.)
Rabbit Heat Shock Protein 27 Polyclonal Antibody | anti-Hsp27 antibody
Heat Shock Protein 27, phosphorylated (Ser82) (HSP27)
Gene Names
Hsp27; 27K; 28; anon-WO0140519.69; CG4466; Dhsp27; DmelCG4466; DmHsp27; hsp 27; hsp-27; hsp26; hsp27; HSP27; hsp27/26; hsp28; Hsp28; HSP28; small hsp locus 67B
Reactivity
Human, Monkey
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity PurifiedPurified by Protein A and peptide affinity chromatography.
Synonyms
Heat Shock Protein 27; Polyclonal Antibody; phosphorylated (Ser82) (HSP27); Anti -Heat Shock Protein 27; anti-Hsp27 antibody
Host
Rabbit
Reactivity
Human, Monkey
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous human HSP27 only when phosphorylated at serine 82. Does not recognize other heat shock proteins.
Purity/Purification
Affinity Purified
Purified by Protein A and peptide affinity chromatography.
Purified by Protein A and peptide affinity chromatography.
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Applicable Applications for anti-Hsp27 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry (ABC).
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1XTBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:50
Immunocytochemistry (ABC): 1:500
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1XTBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:50
Immunocytochemistry (ABC): 1:500
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser82 of human HSP27.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using Phospho-HSP27 (Ser82) Antibody (upper) or control HSP27 antibody (lower). HeLa cells were incubated at 42°C for 0–2 hours as indicated.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using Phospho-HSP27 (Ser82) Antibody (upper) or control HSP27 antibody (lower). HeLa cells were incubated at 42°C for 0–2 hours as indicated.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil, showing cytoplasmic localization, using Phospho-HSP27 (Ser82) Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil, showing cytoplasmic localization, using Phospho-HSP27 (Ser82) Antibody.)
Immunocytochemistry (ICC)
(Immunocytochemical staining of HeLa cells, untreated (left) or UV-treated (right), using anti-Phospho-HSP27 (Ser82) Antibody.)
Immunocytochemistry (ICC)
(Immunocytochemical staining of HeLa cells, untreated (left) or UV-treated (right), using anti-Phospho-HSP27 (Ser82) Antibody.)
Related Product Information for anti-Hsp27 antibody
Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold in order to confer cellular resistance to the adverse environmental change. HSP27 is also phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as the result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been illustrated that phosphorylation and increased concentration of HSP27 modulate actin polymerization and reorganization (5,6).
Product Categories/Family for anti-Hsp27 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
heat shock protein 27
NCBI Official Synonym Full Names
Heat shock protein 27
NCBI Official Symbol
Hsp27
NCBI Official Synonym Symbols
27K; 28; anon-WO0140519.69; CG4466; Dhsp27; DmelCG4466; DmHsp27; hsp 27; hsp-27; hsp26; hsp27; HSP27; hsp27/26; hsp28; Hsp28; HSP28; small hsp locus 67B
NCBI Protein Information
CG4466 gene product from transcript CG4466-RA; heat shock protein 27; CG4466-PA; Hsp27-PA; heat shock protein hsp27; Hsp 27
Protein Family
Research Articles on Hsp27
Similar Products
Product Notes
The Hsp27 (Catalog #AAA629503) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Heat Shock Protein 27, phosphorylated (Ser82) (HSP27) reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Heat Shock Protein 27 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry (ABC). Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1XTBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50 Immunocytochemistry (ABC): 1:500. Researchers should empirically determine the suitability of the Hsp27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock Protein 27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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