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Typical Testing Data/Standard Curve (for reference only)

Mouse Omega-3 fatty acid receptor 1 ELISA Kit | O3far1 elisa kit

Mouse Omega-3 fatty acid receptor 1 ELISA Kit

Gene Names
Ffar4; GT01; Pgr4; Gpr120; Gpr129; O3far1; KPG_013; AI552415
Reactivity
Mouse
Synonyms
Omega-3 fatty acid receptor 1; Mouse Omega-3 fatty acid receptor 1 ELISA Kit; O3far1 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay recognizes recombinant and natural mouse O3FAR1. No significant cross-reactivity or interference was observed.
Sequence Length
361
Assay Type
Sandwich
Detection Range
0.78-50 ng/mL
Sensitivity
0.45 ng/mL
Intra-Assay CV
<=6.9%
Inter-Assay CV
<=8.6%
Recovery
93%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for O3far1 elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to O3FAR1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for O3FAR1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain O3FAR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of O3FAR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,813 Da
NCBI Official Full Name
free fatty acid receptor 4
NCBI Official Synonym Full Names
free fatty acid receptor 4
NCBI Official Symbol
Ffar4
NCBI Official Synonym Symbols
GT01; Pgr4; Gpr120; Gpr129; O3far1; KPG_013; AI552415
NCBI Protein Information
free fatty acid receptor 4
UniProt Protein Name
Free fatty acid receptor 4
UniProt Gene Name
Ffar4
UniProt Synonym Gene Names
Gpr120; O3far1
UniProt Entry Name
FFAR4_MOUSE

Uniprot Description

GPR120: Receptor for medium and long-chain free fatty acid (FAA). Signals via a G(q)/G(11)-coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti- inflammatory effects particularly in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2)/TAB1 dependent effect but independent of G(q)/G(11)-coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. May mediate the taste of fatty acids. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Lipid-binding; Receptor, GPCR; Membrane protein, integral; GPCR, family 1

Cellular Component: endocytic vesicle; integral to membrane; integral to plasma membrane; membrane; plasma membrane

Molecular Function: fatty acid binding; G-protein coupled receptor activity; lipid binding; protein binding; signal transducer activity; taste receptor activity

Biological Process: fat cell differentiation; G-protein coupled receptor protein signaling pathway; hormone secretion; negative regulation of apoptosis; negative regulation of cytokine secretion; negative regulation of inflammatory response; signal transduction

Research Articles on O3far1

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Product Notes

The Mouse O3far1 ffar4 (Catalog #AAA288067) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA288067 ELISA Kit recognizes Mouse O3far1. It is sometimes possible for the material contained within the vial of "Omega-3 fatty acid receptor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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