SDS-PAGE
ANP32A / PHAP1 recombinant protein
Recombinant Human ANP32A / PHAP1 Protein (His & GST tag)
Gene Names
ANP32A; LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
Purity
> 85 % as determined by SDS-PAGE
Synonyms
ANP32A / PHAP1; Recombinant Human ANP32A / PHAP1 Protein (His & GST tag); C15orf1; I1PP2A; LANP; MAPM; MGC119787; MGC150373; PHAP1; PHAPI; PP32; ANP32A / PHAP1 recombinant protein
Host
E Coli
Purity/Purification
> 85 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 10% glycerol, 1mM DTT, 0.5mM GSH
Sequence Length
249
Application Notes
The recombinant human ANP32A/GST chimera consists of 479 amino acids and has a calculated molecular mass of 55.4 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal
Met
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for ANP32A / PHAP1 recombinant protein
Background: acidic leucine-rich nuclear phosphoprotein 32 family member A, also known as acidic nuclear phosphoprotein pp32, Leucine-rich acidic nuclear protein, Mapmodulin, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, Putative HLA-DR-associated protein I, PHAPI and ANP32A, is a nucleus, cytoplasm and endoplasmic reticulum. ANP32A / LANP is expressed in all tissues tested. It is highly expressed in kidney and skeletal muscle, moderate levels of expression is in brain, placenta and pancreas. ANP32A / LANP is weakly expressed in lung. It is found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala. ANP32A / LANP is a component of the SET complex, which also contains SET, APEX1, HMGB2 and NME1. It directly interacts with SET. ANP32A / LANP also interacts with ATXN1/SCA1. ANP32A / LANP is implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. ANP32A / LANP plays a role in E4F1-mediated transcriptional repression.
Description: A DNA sequence encoding the of human ANP32A (NP_006296.1) (Glu 2-Lys 238) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Description: A DNA sequence encoding the of human ANP32A (NP_006296.1) (Glu 2-Lys 238) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,585 Da
NCBI Official Full Name
acidic leucine-rich nuclear phosphoprotein 32 family member A
NCBI Official Synonym Full Names
acidic nuclear phosphoprotein 32 family member A
NCBI Official Symbol
ANP32A
NCBI Official Synonym Symbols
LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
NCBI Protein Information
acidic leucine-rich nuclear phosphoprotein 32 family member A
UniProt Protein Name
Acidic leucine-rich nuclear phosphoprotein 32 family member A
UniProt Gene Name
ANP32A
UniProt Synonym Gene Names
C15orf1; LANP; MAPM; PHAP1; pp32; LANP; PHAPI
Uniprot Description
ANP32A: Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase- independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1- mediated transcriptional repression. Belongs to the ANP32 family.
Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 15q23
Cellular Component: cytoplasm; endoplasmic reticulum; nucleoplasm; nucleus; perinuclear region of cytoplasm
Molecular Function: histone binding; protein binding
Biological Process: histone exchange; nucleocytoplasmic transport; nucleosome assembly; regulation of apoptosis; regulation of mRNA stability
Research Articles on ANP32A / PHAP1
Similar Products
Product Notes
The ANP32A / PHAP1 anp32a (Catalog #AAA2545629) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The recombinant human ANP32A/GST chimera consists of 479 amino acids and has a calculated molecular mass of 55.4 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the ANP32A / PHAP1 anp32a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANP32A / PHAP1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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