Rat Atrial Natriuretic Peptide ELISA Kit | ANP elisa kit

Rat Atrial Natriuretic Peptide ELISA Kit

Cat. Num. AAA765980

• Gene Names: NPPA; ANF; ANP; CDD; CDP; PND; ATFB6; ATRST2; CDD-ANF
• Reactivity: Rat
• Synonyms: Atrial Natriuretic Peptide; Rat Atrial Natriuretic Peptide ELISA Kit; ANP/NPPA/ANF/Cardionatrin/NPPA/PND/ANF/ANPatriopeptin/ATFB6/atrial natriuretic factor/Atrial natriuretic peptide/CDD-ANF/natriuretic peptide A/natriuretic peptide precursor A/PNDcardionatrin/prepronatriodilatin/Prepronatriodilatin; ANP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of ANP. No significant cross-reactivity or interference between ANP and analogues is observed.
• Sequence Length: 151

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 15.625-1000pg/ml
• Sensitivity: <9.375pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ANP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ANP were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Standard Curve/Testing Data

Related Product Information for ANP elisa kit

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with ANP. During the reaction, ANP in the sample or standard competes with a fixed amount of ANP on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to ANP. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ANP in the samples is then determined by comparing the O D of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 178638
• NCBI GeneID: 4878
• Molecular Weight: 16,708 Da
• NCBI Official Full Name: atrial natriuretic peptide
• NCBI Official Synonym Full Names: natriuretic peptide A
• NCBI Official Symbol: NPPA
• NCBI Official Synonym Symbols: ANF; ANP; CDD; CDP; PND; ATFB6; ATRST2; CDD-ANF
• NCBI Protein Information: natriuretic peptides A
• UniProt Protein Name: Natriuretic peptides A
• Protein Family: Andropin
• UniProt Gene Name: NPPA
• UniProt Synonym Gene Names: ANP; PND; CDD; CDP; ANF; ANP
• UniProt Entry Name: ANF_HUMAN

NCBI Description

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1. [provided by RefSeq, Oct 2015]

Uniprot Description

NPPA: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Defects in NPPA are the cause of familial atrial fibrillation type 6 (ATFB6). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity, progressive deterioration of atrial electromechanical function and ineffective pumping of blood into the ventricles. It can be associated with palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the natriuretic peptide family.

Protein type: Secreted; Hormone; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: extracellular region; extracellular space; mast cell granule; nucleus; perinuclear region of cytoplasm

Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; protein binding; receptor binding

Biological Process: cardiac muscle hypertrophy; cellular protein metabolic process; cGMP biosynthetic process; female pregnancy; gene expression; negative regulation of cell growth; negative regulation of systemic arterial blood pressure; neuropeptide signaling pathway; positive regulation of heart rate; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; regulation of blood vessel size; response to hypoxia; response to insulin stimulus; transcription initiation from RNA polymerase II promoter

Disease: Atrial Fibrillation, Familial, 6; Atrial Standstill 2