Canine Angiopoietin 2 ELISA Kit | ANG2 elisa kit

Canine Angiopoietin 2 ELISA Kit

Cat. Num. AAA737439

• Gene Names: ANGPT2; ANG2; AGPT2
• Reactivity: Canine
• Synonyms: Angiopoietin 2; Canine Angiopoietin 2 ELISA Kit; ANG2 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ANG2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ANG2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANG2 elisa kit

Principle of the Assay: ANG2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANG2 antibody and an ANG2-HRP conjugate. The assay sample and buffer are incubated together with ANG2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANG2 concentration since ANG2 from samples and ANG2-HRP conjugate compete for the anti-ANG2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANG2 from the sample, fewer sites are left to bind ANG2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANG2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ANG2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 169646754
• NCBI GeneID: 285
• NCBI Accession #: NP_001112360.1
• NCBI GenBank Nucleotide #: NM_001118888.1
• Molecular Weight: 56,848 Da
• NCBI Official Full Name: angiopoietin-2 isoform c
• NCBI Official Synonym Full Names: angiopoietin 2
• NCBI Official Symbol: ANGPT2
• NCBI Official Synonym Symbols: ANG2; AGPT2
• NCBI Protein Information: angiopoietin-2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a
• UniProt Protein Name: Angiopoietin-2
• Protein Family: Angiogenin
• UniProt Gene Name: ANGPT2
• UniProt Synonym Gene Names: ANG-2
• UniProt Entry Name: ANGP2_HUMAN

NCBI Description

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ANGPT2: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular space; cell projection; extracellular region; plasma membrane; nucleus

Molecular Function: protein binding; metal ion binding; receptor tyrosine kinase binding; receptor binding

Biological Process: Tie receptor signaling pathway; organ regeneration; maternal process involved in pregnancy; negative regulation of blood vessel endothelial cell migration; signal transduction; response to organic cyclic substance; germ cell development; negative regulation of positive chemotaxis; response to radiation; negative regulation of angiogenesis; positive regulation of angiogenesis; response to mechanical stimulus; response to hypoxia; response to glucose stimulus; angiogenesis; response to activity; blood coagulation; leukocyte migration