Western Blot (WB)
(Western blot analysis of extracts from MDA-MB-468 cells, untreated or treated with EGF, using Phospho-EGF Receptor (Tyr 1173) (upper) or EGF Receptor Antibody (lower). This assay serves as a control for the efficacy of the EGF treatment.)
Epidermal Growth Factor Receptor Immunoglobulin
Epidermal Growth Factor Receptor, phosphorylated (Tyr1173) (erbB, EGFR), Immunohistochemistry Controls
Applications
Immunohistochemistry
Synonyms
Epidermal Growth Factor Receptor; phosphorylated (Tyr1173) (erbB; EGFR); Immunohistochemistry Controls; Epidermal Growth Factor Receptor immunoglobulin
Applicable Applications for Epidermal Growth Factor Receptor immunoglobulin
Immunohistochemistry (IHC)
Application Notes
These slides are intended for use in immunohistochemical assays.
Preparation and Storage
Store at 4 degree C. Optimal staining is achieved if slides are stained following our standard IHC protocols and are used within 8 weeks of assay date; however, signals may persist beyond two months.
Western Blot (WB)
(Western blot analysis of extracts from MDA-MB-468 cells, untreated or treated with EGF, using Phospho-EGF Receptor (Tyr 1173) (upper) or EGF Receptor Antibody (lower). This assay serves as a control for the efficacy of the EGF treatment.)
Western Blot (WB)
(Western blot analysis of extracts from MDA-MB-468 cells, untreated or treated with EGF, using Phospho-EGF Receptor (Tyr 1173) (upper) or EGF Receptor Antibody (lower). This assay serves as a control for the efficacy of the EGF treatment.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded MDA-MB-468 cells, untreated (left) or treated with EGF (right), using Phospho-EGF Receptor.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded MDA-MB-468 cells, untreated (left) or treated with EGF (right), using Phospho-EGF Receptor.)
Related Product Information for Epidermal Growth Factor Receptor immunoglobulin
The epidermal growth factor (EGF) receptor is a 170kD transmembrane tyrosine kinase and member of the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLC binds at phospho-Tyr992, resulting in activation of PLC -mediated downstream signaling (6). Phosphorylation of Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated residues (Tyr1148 and Tyr1173) provide a docking site for the SHC scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutations to either of these serines upregulate EGFR tyrosine autokinase activity (10).
Sections are cut freshly upon ordering.
Each control slide contains formalin fixed, paraffin-embedded MDA-MB-468 cells, both untreated and treated with EGF, that serve as a control for Phospho-EGFR (Tyr1173) immunostaining. Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the EGF treatment.
Sections are cut freshly upon ordering.
Each control slide contains formalin fixed, paraffin-embedded MDA-MB-468 cells, both untreated and treated with EGF, that serve as a control for Phospho-EGFR (Tyr1173) immunostaining. Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the EGF treatment.
Product Categories/Family for Epidermal Growth Factor Receptor immunoglobulin
Similar Products
Product Notes
The Epidermal Growth Factor Receptor (Catalog #AAA652639) is an Immunoglobulin and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Epidermal Growth Factor Receptor can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). These slides are intended for use in immunohistochemical assays. Researchers should empirically determine the suitability of the Epidermal Growth Factor Receptor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Epidermal Growth Factor Receptor, Immunoglobulin" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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