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AMY2A / Alpha-amylase Recombinant Protein | AMY2A recombinant protein

Recombinant Human AMY2A / Alpha-amylase Protein (His tag)

Gene Names
AMY2A; PA; AMY2
Purity
> 85 % as determined by SDS-PAGE
Synonyms
AMY2A / Alpha-amylase; Recombinant Human AMY2A / Alpha-amylase Protein (His tag); AMY2A; AMY2A recombinant protein
Ordering
For Research Use Only!
Host
Human Cells
Purity/Purification
> 85 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
511
Application Notes
The recombinant human AMY2A consists of 507 amino acids and predicts a molecular mass of 57.3 KDa. It migrates as an approximately 53-58 KDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal
Gln 16
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
Related Product Information for AMY2A recombinant protein
Background: Alpha-amylase is the major form of amylase found in humans and other mammals. Amylases are secreted proteins that hydrolyze 1, 4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. Alpha-amylase hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Amylases is widely expressed and is most prominent in pancreatic juice and saliva, each of which has its own isoform of human alpha-amylase. They behave differently on isoelectric focusing, and can also be separated in testing by using specific monoclonal antibodies.

Description: A DNA sequence encoding the human AMY2A (P04746) (Met1-Leu511) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
279
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,302 Da
NCBI Official Full Name
pancreatic alpha-amylase
NCBI Official Synonym Full Names
amylase, alpha 2A (pancreatic)
NCBI Official Symbol
AMY2A
NCBI Official Synonym Symbols
PA; AMY2
NCBI Protein Information
pancreatic alpha-amylase
UniProt Protein Name
Pancreatic alpha-amylase
UniProt Gene Name
AMY2A
UniProt Synonym Gene Names
PA

NCBI Description

This gene encodes a member of the alpha-amylase family of proteins. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen. This gene and several family members are present in a gene cluster on chromosome 1. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jan 2015]

Uniprot Description

AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

Protein type: Carbohydrate Metabolism - starch and sucrose; EC 3.2.1.1; Hydrolase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p21.1

Cellular Component: extracellular region; extracellular space

Molecular Function: alpha-amylase activity; calcium ion binding; chloride ion binding

Biological Process: carbohydrate catabolic process; polysaccharide digestion

Research Articles on AMY2A

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Product Notes

The AMY2A amy2a (Catalog #AAA2545341) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant human AMY2A consists of 507 amino acids and predicts a molecular mass of 57.3 KDa. It migrates as an approximately 53-58 KDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the AMY2A amy2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMY2A / Alpha-amylase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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