Immunohistochemistry (IHC)
(Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
Mouse anti-Human AMY2A/Pancreatic Amylase Monoclonal Antibody | anti-AMY2A antibody
Anti-AMY2A/Pancreatic Amylase Antibody (aa135-408, clone 1E3)
Gene Names
AMY2A; PA; AMY2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
AMY2A/Pancreatic Amylase; Monoclonal Antibody; Anti-AMY2A/Pancreatic Amylase Antibody (aa135-408; clone 1E3); Mouse Monoclonal [clone 6D4] (IgG2b) to Human AMY2A/Pancreatic Amylase; AMY2A; Alpha-amylase; Amylase; alpha 2A (pancreatic); Found in the pancreas; Glycogenase; Pancreatic alpha-amylase; Pancreatic Amylase; Pancreatic amylase 2A; AMY2; alpha 2A pancreatic; pancreatic; alpha-2A; Pancreatic amylase alpha 2A; Pa; anti-AMY2A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1E3
Specificity
Human AMY2A/Pancreatic Amylase
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Sequence Length
511
Applicable Applications for anti-AMY2A antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 10 ug/ml
WB: 1:2000
WB: 1:2000
Immunogen
Human recombinant protein fragment corresponding to amino acids 135-408 of human AMY2A (NP_000690) produced in E. coli.
Conjugation
Unconjugated
Epitope
aa135-408
Preparation and Storage
Store at -20 degree C.
Avoid freeze-thaw cycles.
Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
Western Blot (WB)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMY2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMY2A.)
Western Blot (WB)
(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMY2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMY2A.)
Related Product Information for anti-AMY2A antibody
Amylases are secreted proteins that hydrolyze 1, 4-alpha-glucoside bondsin oligosaccharides and polysaccharides, and thus catalyze the firststep in digestion of dietary starch and glycogen. the human genome has acluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylaseiso enzyme produced by the pancreas.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,302 Da
NCBI Official Full Name
pancreatic alpha-amylase
NCBI Official Synonym Full Names
amylase, alpha 2A (pancreatic)
NCBI Official Symbol
AMY2A
NCBI Official Synonym Symbols
PA; AMY2
NCBI Protein Information
pancreatic alpha-amylase
UniProt Protein Name
Pancreatic alpha-amylase
UniProt Gene Name
AMY2A
UniProt Synonym Gene Names
PA
NCBI Description
This gene encodes a member of the alpha-amylase family of proteins. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, catalyzing the first step in digestion of dietary starch and glycogen. This gene and several family members are present in a gene cluster on chromosome 1. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jan 2015]
Research Articles on AMY2A
Similar Products
Product Notes
The AMY2A amy2a (Catalog #AAA2401346) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-AMY2A/Pancreatic Amylase Antibody (aa135-408, clone 1E3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AMY2A/Pancreatic Amylase can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 10 ug/ml WB: 1:2000. Researchers should empirically determine the suitability of the AMY2A amy2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMY2A/Pancreatic Amylase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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