Alpha 1-Microglobulin/AMBP Recombinant Protein | AMBP recombinant protein

Recombinant Human Alpha 1-Microglobulin/AMBP Protein (C-His)

Cat. Num. AAA2557381

• Gene Names: AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Alpha 1-Microglobulin/AMBP; Recombinant Human Alpha 1-Microglobulin/AMBP Protein (C-His); Protein AMBP; Alpha-1-Microglobulin; Protein HC; Alpha-1 Microglycoprotein; Complex-Forming Glycoprotein Heterogeneous in Charge; Inter-Alpha-Trypsin Inhibitor Light Chain; ITI-LC; Bikunin; EDC1; HI-30; Uronic-Acid-Rich Protein; Trypstatin; AMBP; HCP; ITIL; AMBP recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH7.4.
• Sequence: Gly20-Val203
• Sequence Length: 352

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for AMBP recombinant protein

Protein Construction: Recombinant Human Alpha 1-Microglobulin is produced by our mammalian expression system and the target gene encoding Gly20-Val203 is expressed with a 6His tag at the C-terminus.

Background: Protein AMBP belongs to the calycin superfamily and Lipocalin family. AMBP can be cleaved into three chains: alpha-1-microglobulin, inter-alpha-trypsin inhibitor light chain and trypstatin. AMBP is expressed by the liver and secreted in plasma. alpha-1-microglobulin occurs in many physiological fluids including the plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in the plasma and urine. alpha-1-microglobulin occurs as a monomer and also in complexes with IgA and albumin, Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin and lysosomal granulocytic elastase. Trypstatin act as a trypsin inhibitor, exists in a monomer forms and also occurs as a complex with tryptase in mast cells.

NCBI and Uniprot Product Information

• NCBI GI #: 122801
• NCBI GeneID: 259
• UniProt Accession #: P02760
• Molecular Weight: Molecular Mass: 21.9kDa; Actual Protein Molecular Mass: 33kDa
• NCBI Official Full Name: Protein AMBP
• NCBI Official Synonym Full Names: alpha-1-microglobulin/bikunin precursor
• NCBI Official Symbol: AMBP
• NCBI Official Synonym Symbols: A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
• NCBI Protein Information: protein AMBP
• UniProt Protein Name: Protein AMBP
• Protein Family: Protein
• UniProt Gene Name: AMBP
• UniProt Synonym Gene Names: HCP; ITIL; Protein HC; ITI-LC
• UniProt Entry Name: AMBP_HUMAN

NCBI Description

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008]

Uniprot Description

AMBP: Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q32-q33

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; calcium oxalate binding; IgA binding; heme binding; calcium channel inhibitor activity

Biological Process: negative regulation of JNK cascade; receptor-mediated endocytosis; viral reproduction; heme catabolic process; negative regulation of immune response; protein catabolic process; female pregnancy; cell adhesion; protein-chromophore linkage