Canine alpha Smooth Muscle Actin ELISA Kit | alpha-SMA elisa kit

Canine alpha Smooth Muscle Actin ELISA Kit

Cat. Num. AAA19940

• Gene Names: ACTG2; ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
• Reactivity: Canine
• Synonyms: alpha Smooth Muscle Actin; Canine alpha Smooth Muscle Actin ELISA Kit; alpha-SMA elisa kit

• Reactivity: Canine
• Specificity: No significant cross-reactivity or interference between Canine alpha-SMA and analogues was observed.
• Sequence Length: 376

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 31.2 pg/ml - 1000 pg/ml.
• Sensitivity: 5.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated alpha-SMA concentrations in Canine serum, plasma and other body fluids. Using Purified Canine alpha-SMA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add alpha-SMA and alpha-SMA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of alpha-SMA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for alpha-SMA elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated alpha-SMA concentrations in Canine serum, plasma and other body fluids. Using Purified Canine alpha-SMA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add alpha-SMA and alpha-SMA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of alpha-SMA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4501889
• NCBI GeneID: 72
• NCBI Accession #: NP_001606.1
• NCBI GenBank Nucleotide #: NM_001615.3
• UniProt Accession #: P63267; P12718; Q504R1; Q6FI22; B2R7E7; B4E315; D6W5H8; E9PG30
• Molecular Weight: 41,877 Da
• NCBI Official Full Name: actin, gamma-enteric smooth muscle isoform 1
• NCBI Official Synonym Full Names: actin, gamma 2, smooth muscle, enteric
• NCBI Official Symbol: ACTG2
• NCBI Official Synonym Symbols: ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
• NCBI Protein Information: actin, gamma-enteric smooth muscle; alpha-actin-3; actin-like protein
• UniProt Protein Name: Actin, gamma-enteric smooth muscle
• UniProt Gene Name: ACTG2
• UniProt Synonym Gene Names: ACTA3; ACTL3; ACTSG
• UniProt Entry Name: ACTH_HUMAN

NCBI Description

Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010]

Uniprot Description

ACTG2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Contractile

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: extracellular space; cytoskeleton; cytosol

Molecular Function: ATP binding

Biological Process: muscle contraction

Disease: Visceral Myopathy