ApoA1 elisa kit

Human Apolipoprotein A1 ELISA Kit

Cat. Num. AAA135999

• Gene Names: APOA1; apo(a)
• Synonyms: ApoA1; Human Apolipoprotein A1 ELISA Kit; ApoA1 elisa kit

• Specificity: This assay has high sensitivity and excellent specificity for detection of HTR2A. No significant cross-reactivity or interference between HTR2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HTR2A and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 14

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store at 4 degree C

Testing Data

Related Product Information for ApoA1 elisa kit

Intended Uses: This HTR2A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HTR2A. This ELISA kit for research use only!

Principle of the Assay: HTR2A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HTR2A antibody and an HTR2A-HRP conjugate. The assay sample and buffer are incubated together with HTR2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HTR2A concentration since HTR2A from samples and HTR2A-HRP conjugate compete for the anti-HTR2A antibody binding site. Since the number of sites is limited, as more sites are occupied by HTR2A from the sample, fewer sites are left to bind HTR2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HTR2A concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ApoA1 elisa kit

HUMAN PLASMA PROTEINS; Human Kits; Plasma Protein ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 553183
• NCBI GeneID: 335
• Molecular Weight: 30,778 Da
• NCBI Official Full Name: APOA1, partial
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: APOA1
• NCBI Official Synonym Symbols: apo(a)
• NCBI Protein Information: apolipoprotein A-I
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I
• UniProt Entry Name: APOA1_HUMAN

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Cell development/differentiation; Endoplasmic reticulum; Lipid-binding; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 11q23-q24

Cellular Component: chylomicron; cytoplasmic vesicle; cytosol; early endosome; endocytic vesicle; endoplasmic reticulum lumen; extracellular region; extracellular space; plasma membrane

Molecular Function: apolipoprotein A-I receptor binding; apolipoprotein receptor binding; beta-amyloid binding; chemorepellent activity; cholesterol binding; cholesterol transporter activity; enzyme binding; identical protein binding; phosphatidylcholine binding; phospholipid binding; protein binding

Biological Process: cellular protein metabolic process; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; G-protein coupled receptor protein signaling pathway; integrin-mediated signaling pathway; lipoprotein biosynthetic process; lipoprotein metabolic process; negative chemotaxis; negative regulation of cytokine secretion during immune response; negative regulation of heterotypic cell-cell adhesion; negative regulation of inflammatory response; negative regulation of interleukin-1 beta secretion; neurite regeneration; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; phospholipid efflux; phospholipid homeostasis; platelet degranulation; positive regulation of fatty acid biosynthetic process; positive regulation of hydrolase activity; positive regulation of lipoprotein lipase activity; positive regulation of Rho protein signal transduction; positive regulation of stress fiber formation; protein amino acid oxidation; protein stabilization; receptor-mediated endocytosis; regulation of Cdc42 protein signal transduction; regulation of cholesterol absorption; retinoid metabolic process; reverse cholesterol transport; transforming growth factor beta receptor signaling pathway; transmembrane transport; triacylglycerol catabolic process; vitamin transport

Disease: Amyloidosis, Familial Visceral; Hypoalphalipoproteinemia, Primary