Chitinase 3-Like 1  Recombinant Protein | CHI3L1 recombinant protein

Recombinant Human Chitinase 3-Like 1 

Cat. Num. AAA146347

• Gene Names: ALKBH3; ABH3; PCA1; DEPC1; DEPC-1
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Chitinase 3-Like 1 ; Recombinant Human Chitinase 3-Like 1 ; CHI3L1 Human; Chitinase 3-Like 1 Human Recombinant; Chitinase 3-like 1; CHI3L1; Chitinase-3-like protein 1; 39 kDa synovial protein; Cartilage glycoprotein 39; CGP-39; GP-39; hCGP-39; CHI3L1 recombinant protein

• Host: Chinese Hamster Ovarian Cells (CHO)
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The CHI3L1 solution (0.25mg/ml) contains Phosphate-Buffered Saline (pH 7.4).; Sterile Filtered colorless solution.
• Sequence: MGVKASQTGF VVLVLLQCCS AYKLVCYYTS WSQYREGDGS CFPDALDRFL CTHIIYSFAN ISNDHIDTWE WNDVTLYGML NTLKNRNPNL KTLLSVGGWN FGSQRFSKIA SNTQSRRTFI KSVPPFLRTH GFDGLDLAWL YPGRRDKQHF TTLIKEMKAE FIKEAQPGKK QLLLSAALSA GKVTIDSSYD IAKISQHLDF ISIMTYDFHG AWRGTTGHHS PLFRGQEDAS PDRFSNTDYA VGYMLRLGAP ASKLVMGIPT FGRSFTLASS ETGVGAPISG PGIPGRFTKE AGTLAYYEIC DFLRGATVHR ILGQQVPYAT KGNQWVGYDD QESVKSKVQY LKDRQLAGAM VWALDLDDFQ GSFCGQDLRF PLTNAIKDAL AATKLGPEQK LISEEDLNSA VDHHHHHH .
• Sequence Length: 286

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for CHI3L1 recombinant protein

Description: CHI3L1 Human Recombinant produced in CHO is a single, glycosylated polypeptide chain containing 408 amino acids (1-383) and having a molecular mass of 45.5kDa. CHI3L1 is fused to a 25 amino acid myc-His-tag at C-terminus & purified by proprietary chromatographic techniques.

Introduction: Chitinase 3-Like 1 (CHI3L1) catalyze the hydrolysis of chitin that is an abundant glycopolymer present in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases comprises 8 human family members. CHI3L1 belongs to the glycosyl hydrolase 18 family. CHI3L1 lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. CHI3L1 takes part in the process of inflammation and tissue remodeling.

Product Categories/Family for CHI3L1 recombinant protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 21040275
• NCBI GeneID: 221120
• NCBI Accession #: NP_631917.1
• NCBI GenBank Nucleotide #: NM_139178.3
• UniProt Accession #: Q96Q83; Q3SYI0; Q6NX57; Q96BU8; A6NDJ1
• Molecular Weight: 19,310 Da
• NCBI Official Full Name: alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
• NCBI Official Synonym Full Names: alkB, alkylation repair homolog 3 (E. coli)
• NCBI Official Symbol: ALKBH3
• NCBI Official Synonym Symbols: ABH3; PCA1; DEPC1; DEPC-1
• NCBI Protein Information: alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; alkylated DNA repair protein alkB homolog 3; prostate cancer antigen 1; prostate cancer antigen-1
• UniProt Protein Name: Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3
• Protein Family: Chitinase-3-like protein
• UniProt Gene Name: ALKBH3
• UniProt Synonym Gene Names: ABH3; DEPC1
• UniProt Entry Name: ALKB3_HUMAN

NCBI Description

The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]

Uniprot Description

ALKBH3: Dioxygenase that repairs alkylated DNA containing 1- methyladenine (1meA) and 3-methylcytosine (3meC) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated 3mC within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Belongs to the alkB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Demethylase; EC 1.14.11.-; Hydrolase; DNA repair, damage

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: protein binding; L-ascorbic acid binding; ferrous iron binding; DNA-N1-methyladenine dioxygenase activity; DNA demethylase activity

Biological Process: cell proliferation; DNA dealkylation; DNA repair