ALDOB recombinant protein

ALDOB; human recombinant

Cat. Num. AAA847085

• Gene Names: ALDOB; ALDB; ALDO2
• Applications: SDS-Page
• Purity: > 95%
• Synonyms: ALDOB; human recombinant; Fructose-bisphosphate aldolase B; ALDB; ALDO2; ALDOB recombinant protein

• Host: E Coli; Source: Human
• Purity/Purification: > 95%
• Form/Format: Liquid; In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT; 10% glycerol; 0.1 M NaCl
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSH DSSINQSIGG VILFHETLYQ KDSQGKLFRN ILKEKGIVVG IKLDQGGAPL AGTNKETTIQ GLDGLSERCA QYKKDGVDFG KWRAVLRIAD QCPSSLAIQE NANALARYAS ICQQNGLVPI VEPEVIPDGD HDLEHCQYVT EKVLAAVYKA LNDHHVYLEG TLLKPNMVTA GHACTKKYTP MAHRFP ALTQEQKKEL SEIAQSIVAN GKGILAADES VGTMGNRLQR IKVENTEENR RQFREILFSV EQVAMATVTA LHRTVPAAVP GICFLSGGMS EEDATLNLNA INLCPLPKPW KLSFSYGRAL QASALAAWGG KAANKEATQE AFMKRAMANC QAAKGQYVHT GSSGAASTQS LFTACYT
• Sequence Length: 364

• Applicable Applications for ALDOB recombinant protein: SDS-PAGE
• Preparation and Storage: Store at -20 degree C for 12 months.; Centrifuge the vial prior to opening.

Testing Data

Related Product Information for ALDOB recombinant protein

Fructose-bisphosphate aldolase B; also known as ALDOB; is one of three known aldolase isoenzymes; and is found in kidney and small adult intestine where it is associated with aldolases A or C. ALDOB catalyzes the reversible cleavage of fructose 1-phosphate into dihydroxyacetone phosphate and glyceraldehyde. It is regulated by the hormones Insulin and glucagon and has been implicated in hereditary fructose intolerance disease. Recombinant human ALDOB protein; fused to His-tag at N-terminus; was expressed in E Coli and purified by using conventional chromatography techniques.

NCBI and Uniprot Product Information

• NCBI GI #: 40354205
• NCBI GeneID: 229
• NCBI Accession #: NP_000026.2
• NCBI GenBank Nucleotide #: NM_000035.3
• UniProt Accession #: P05062; Q13741; Q13742; Q5T7D6
• Molecular Weight: This protein is fused with 6x his tag at N Terminus and the protein has a calculated MW of 42 kDa (388aa)
• NCBI Official Full Name: fructose-bisphosphate aldolase B
• NCBI Official Synonym Full Names: aldolase, fructose-bisphosphate B
• NCBI Official Symbol: ALDOB
• NCBI Official Synonym Symbols: ALDB; ALDO2
• NCBI Protein Information: fructose-bisphosphate aldolase B
• UniProt Protein Name: Fructose-bisphosphate aldolase B
• Protein Family: Fructose-bisphosphate aldolase
• UniProt Gene Name: ALDOB
• UniProt Synonym Gene Names: ALDB

NCBI Description

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008]

Uniprot Description

ALDOB: Defects in ALDOB are the cause of hereditary fructose intolerance (HFI). HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. Belongs to the class I fructose-bisphosphate aldolase family.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 4.1.2.13; Lyase

Chromosomal Location of Human Ortholog: 9q31.1

Cellular Component: cytosol; microtubule organizing center

Molecular Function: ATPase binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; identical protein binding; protein binding

Biological Process: fructose 1,6-bisphosphate metabolic process; fructose metabolic process; gluconeogenesis; glycolysis; NADH oxidation; positive regulation of ATPase activity

Disease: Fructose Intolerance, Hereditary