ALDOB recombinant protein

ALDOB, 1-364aa, Human, His tag, E Coli

Cat. Num. AAA204182

• Gene Names: ALDOB; ALDB; ALDO2
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: ALDOB; 1-364aa; Human; His tag; E Coli; Fructose-bisphosphate aldolase B; ALDB; ALDO2; Fructose bisphosphate aldolase B; ALDOB recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAHRFP ALTQEQKKEL SEIAQSIVAN GKGILAADES VGTMGNRLQR IKVENTEENR RQFREILFSV DSSINQSIGG VILFHETLYQ KDSQGKLFRN ILKEKGIVVG IKLDQGGAPL AGTNKETTIQ GLDGLSERCA QYKKDGVDFG KWRAVLRIAD QCPSSLAIQE NANALARYAS ICQQNGLVPI VEPEVIPDGD HDLEHCQYVT EKVLAAVYKA LNDHHVYLEG TLLKPNMVTA GHACTKKYTP EQVAMATVTA LHRTVPAAVP GICFLSGGMS EEDATLNLNA INLCPLPKPW KLSFSYGRAL QASALAAWGG KAANKEATQE AFMKRAMANC QAAKGQYVHT GSSGAASTQS LFTACYTY
• Sequence Length: 364

• Applicable Applications for ALDOB recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ALDOB recombinant protein

Fructose-bisphosphate aldolase B, also known as ALDOB, is one of three known aldolase isoenzymes, and is found in kidney and small adult intestine where it is associated with aldolases A or C. ALDOB catalyzes the reversible cleavage of fructose 1-phosphate into dihydroxyacetone phosphate and glyceraldehyde. It is regulated by the hormones Insulin and glucagon and has been implicated in hereditary fructose intolerance disease. Recombinant human ALDOB protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ALDOB recombinant protein

Metabolism

References

Caffe A R., et al. (1994) J Comp Neurol. 348:291-297.; Dehnes Y., et al. (1998) J Neurosci. 18:3606-3619.

NCBI and Uniprot Product Information

• NCBI GI #: 40354205
• NCBI GeneID: 229
• NCBI Accession #: NP_000026
• NCBI GenBank Nucleotide #: NM_000035.3
• UniProt Accession #: P05062; Q13741; Q13742; Q5T7D6
• Molecular Weight: 42 kDa (388aa) confirmed by MALDI-TOF
• NCBI Official Full Name: fructose-bisphosphate aldolase B
• NCBI Official Synonym Full Names: aldolase, fructose-bisphosphate B
• NCBI Official Symbol: ALDOB
• NCBI Official Synonym Symbols: ALDB; ALDO2
• NCBI Protein Information: fructose-bisphosphate aldolase B
• UniProt Protein Name: Fructose-bisphosphate aldolase B
• Protein Family: Fructose-bisphosphate aldolase
• UniProt Gene Name: ALDOB
• UniProt Synonym Gene Names: ALDB

NCBI Description

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008]

Uniprot Description

MiscellaneousIn vertebrates, 3 forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.