Rabbit RPS8 Polyclonal Antibody | anti-RPS8 antibody

Anti-RPS8 Antibody

Cat. Num. AAA821714

• Gene Names: RPS8; S8
• Reactivity: Human, Mouse, Rat, Bovine, Chicken, Monkey, Sheep
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: The antibody was purified by immunogen affinity chromatography.
• Synonyms: RPS8; Polyclonal Antibody; Anti-RPS8 Antibody; 40S ribosomal protein S8; anti-RPS8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Chicken, Monkey, Sheep
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of RPS8 protein.
• Purity/Purification: The antibody was purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Sequence Length: 208

• Applicable Applications for anti-RPS8 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RPS8. The exact sequence is proprietary.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of RPS8 expression in HepG2 (A), A431 (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of RPS8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Related Product Information for anti-RPS8 antibody

Rabbit polyclonal antibody to RPS8

NCBI and Uniprot Product Information

• NCBI GI #: 4506743
• NCBI GeneID: 6202
• NCBI Accession #: NP_001003.1
• NCBI GenBank Nucleotide #: NM_001012.1
• UniProt Accession #: P62241; P09058; Q6IRL7
• Molecular Weight: 24,205 Da
• NCBI Official Full Name: 40S ribosomal protein S8
• NCBI Official Synonym Full Names: ribosomal protein S8
• NCBI Official Symbol: RPS8
• NCBI Official Synonym Symbols: S8
• NCBI Protein Information: 40S ribosomal protein S8; OK/SW-cl.83
• UniProt Protein Name: 40S ribosomal protein S8
• Protein Family: 30S ribosomal protein
• UniProt Gene Name: RPS8
• UniProt Entry Name: RS8_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S8E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal tumors and colon polyps compared to matched normal colonic mucosa has been observed. This gene is co-transcribed with the small nucleolar RNA genes U38A, U38B, U39, and U40, which are located in its fourth, fifth, first, and second introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS8: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S8E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal tumors and colon polyps compared to matched normal colonic mucosa has been observed. This gene is co-transcribed with the small nucleolar RNA genes U38A, U38B, U39, and U40, which are located in its fourth, fifth, first, and second introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 1p34.1-p32

Cellular Component: focal adhesion; membrane; cytoplasm; nucleus; cytosol; ribonucleoprotein complex

Molecular Function: structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); translational elongation; cellular protein metabolic process; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; viral infectious cycle; translational termination