Rat Activated Leukocyte Cell Adhesion Molecule ELISA Kit | ALCAM elisa kit

Rat Activated Leukocyte Cell Adhesion Molecule ELISA Kit

Cat. Num. AAA725431

• Gene Names: ALCAM; MEMD; CD166
• Reactivity: Rat
• Synonyms: Activated Leukocyte Cell Adhesion Molecule; Rat Activated Leukocyte Cell Adhesion Molecule ELISA Kit; ALCAM elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This ALAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ALAM. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALCAM elisa kit

Principle of the assay: ALAM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALAM antibody and an ALAM-HRP conjugate. The assay sample and buffer are incubated together with ALAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALAM concentration since ALAM from samples and ALAM-HRP conjugate compete for the anti-ALAM antibody binding site. Since the number of sites is limited, as more sites are occupied by ALAM from the sample, fewer sites are left to bind ALAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALAM concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ALCAM elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 886258
• NCBI GeneID: 214
• Molecular Weight: 33,878 Da
• NCBI Official Full Name: alcam
• NCBI Official Synonym Full Names: activated leukocyte cell adhesion molecule
• NCBI Official Symbol: ALCAM
• NCBI Official Synonym Symbols: MEMD; CD166
• NCBI Protein Information: CD166 antigen
• UniProt Protein Name: CD166 antigen
• Protein Family: CD166 antigen
• UniProt Gene Name: ALCAM
• UniProt Synonym Gene Names: MEMD
• UniProt Entry Name: CD166_HUMAN

NCBI Description

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]

Uniprot Description

ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3q13.1

Cellular Component: focal adhesion; cell soma; axon; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: axon guidance; motor axon guidance; cell adhesion; signal transduction