Aldo-Keto Reductase Family 1 Member D1 Recombinant Protein | AKR1D1 recombinant protein

Recombinant Human Aldo-Keto Reductase Family 1 Member D1

Cat. Num. AAA140966

• Gene Names: AKR1D1; CBAS2; SRD5B1; 3o5bred
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Aldo-Keto Reductase Family 1 Member D1; Recombinant Human Aldo-Keto Reductase Family 1 Member D1; AKR1D1 Human; Aldo-Keto Reductase Family 1 Member D1 Human Recombinant; 3-oxo-5-beta-steroid 4-dehydrogenase; Aldo-Keto Reductase Family 1; Member D1; SRD5B1; Delta(4)-3-Ketosteroid 5-Beta-Reductase; Delta 4-3-Ketosteroid-5-Beta-Reductase; Delta(4)-3-Oxosteroid 5-Beta-Reductase; CBAS2; Steroid-5-Beta-Reductase; Beta Polypeptide 1 (3-Oxo-5 Beta-Steroid Delta 4-Dehydrogenase Beta 1); Member D1 (Delta 4-3-Ketosteroid-5-Beta-Reductase); 3-Oxo-5-Beta-Steroid 4-Dehydrogenase; EC 1.3.1.3; 3o5bred; AKR1D1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: The AKR1D1 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.5), 1mM DTT, 0.1M NaCl and 10% glycerol.; Sterile filtered colorless solution.
• Sequence: MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYRHIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIEVPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELILNKPGLKHKPV SNQVECHPYFTQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERIKENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY
• Sequence Length: 285

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for AKR1D1 recombinant protein

Introduction: Aldo-keto reductase family 1 member D1 (AKR1D1) belongs to the AKR superfamily. The AKR family proteins are soluble NADPH oxidoreductases, which have vital roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is also responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones that carry a delta (4)-3-1 structure. AKR1D1 is highly expressed in the liver, colon and testis. Deficiency of the AKR1D1 enzyme may contribute to hepatic dysfunction.

Description: AKR1D1 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 326 amino acids (1-326 a.a.) and having a molecular mass of 37.3kDa. The AKR1D1 is purified by proprietary chromatographic techniques.

Product Categories/Family for AKR1D1 recombinant protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 300116271
• NCBI GeneID: 6718
• NCBI Accession #: NP_001177835.1
• NCBI GenBank Nucleotide #: NM_001190906.1
• UniProt Accession #: P51857; A1L4P6; A8K060; B4DPN3; B4DPN8
• Molecular Weight: 32,890 Da
• NCBI Official Full Name: 3-oxo-5-beta-steroid 4-dehydrogenase isoform 2
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member D1
• NCBI Official Symbol: AKR1D1
• NCBI Official Synonym Symbols: CBAS2; SRD5B1; 3o5bred
• NCBI Protein Information: 3-oxo-5-beta-steroid 4-dehydrogenase
• UniProt Protein Name: 3-oxo-5-beta-steroid 4-dehydrogenase
• Protein Family: 3-oxo-5-beta-steroid 4-dehydrogenase
• UniProt Gene Name: AKR1D1
• UniProt Synonym Gene Names: SRD5B1
• UniProt Entry Name: AK1D1_HUMAN

Uniprot Description

AKR1D1: Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7- alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4- cholesten-3-one can also act as substrates. Defects in AKR1D1 are the cause of congenital bile acid synthesis defect type 2 (CBAS2); also known as cholestasis with delta(4)-3-oxosteroid 5-beta-reductase deficiency. Patients with this liver disease show absence or low levels of chenodeoxycholic acid and cholic acid in plasma and urine. Belongs to the aldo/keto reductase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.3.1.3; Lipid Metabolism - C21-steroid hormone; Lipid Metabolism - androgen and estrogen; Lipid Metabolism - primary bile acid biosynthesis; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q32-q33

Cellular Component: cytosol

Molecular Function: aldo-keto reductase activity; steroid binding

Biological Process: androgen metabolic process; bile acid biosynthetic process; C21-steroid hormone metabolic process; cholesterol catabolic process; digestion

Disease: Bile Acid Synthesis Defect, Congenital, 2