Typical Testing Data/Standard Curve (for reference only)
Mouse CXC-chemokine receptor 1 ELISA Kit | CXCR1 elisa kit
Mouse CXC-chemokine receptor 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CXCR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CXCR1 antibody and an CXCR1-HRP conjugate. The assay sample and buffer are incubated together with CXCR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CXCR1 concentration since CXCR1 from samples and CXCR1-HRP conjugate compete for the anti-CXCR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CXCR1 from the sample, fewer sites are left to bind CXCR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCR1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq, Jul 2008]
Uniprot Description
IL8RA: Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Receptor, cytokine
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: membrane; plasma membrane; integral to membrane
Molecular Function: G-protein coupled receptor activity; interleukin-8 receptor activity; chemokine receptor activity; interleukin-8 binding
Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; receptor internalization; dendritic cell chemotaxis; chemotaxis; inflammatory response
Disease: Human Immunodeficiency Virus Type 1, Susceptibility To
Research Articles on CXCR1
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Product Notes
The Mouse CXCR1 cxcr1 (Catalog #AAA728400) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA728400 ELISA Kit recognizes Mouse CXCR1. It is sometimes possible for the material contained within the vial of "CXC-chemokine receptor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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