Aryl Hydrocarbon Receptor (AhR) Recombinant Protein | AhR recombinant protein

Recombinant Aryl Hydrocarbon Receptor (AhR)

Cat. Num. AAA2009576

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Aryl Hydrocarbon Receptor (AhR); Recombinant Aryl Hydrocarbon Receptor (AhR); AhR recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-VELCNKGSGY QFIHAADMLH CAESHIRMIK TGESGMTVFR LLAKHSRWRW VQSNARLIYR NGRPDYIIAT QRPLTDEEGR EHLQKRSMTL PFMFATGEAV LYEISSPFSP IMDPLPIRTK SNTSRKDWAP QSTPSKDSFH PNSLMSALIQ QDESIYLCPP SSPAPLDSHF LMDSMSECGS WQGSFAVASN EALLKHEEIR HTQDVNLTLS GGPSELFPDN KNNDLYSIMR NLGIDFEDIR SMQNEEFFRT DSSGEVDFKD IDITDEILT
• Sequence Length: 853

• Applicable Applications for AhR recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Val311~Thr579 (Accession # P41738) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 145207984
• NCBI GeneID: 25690
• NCBI Accession #: NP_037281.2
• NCBI GenBank Nucleotide #: NM_013149.2
• UniProt Accession #: P41738; O88930; O89105
• Molecular Weight: 32.1kDa
• NCBI Official Full Name: aryl hydrocarbon receptor
• NCBI Official Synonym Full Names: aryl hydrocarbon receptor
• NCBI Official Symbol: Ahr
• NCBI Protein Information: aryl hydrocarbon receptor; ah receptor
• UniProt Protein Name: Aryl hydrocarbon receptor
• Protein Family: Aldehyde reductase
• UniProt Gene Name: Ahr
• UniProt Synonym Gene Names: Ah receptor; AhR
• UniProt Entry Name: AHR_RAT

NCBI Description

binds 3-methylcholanthrene and other aromatic hydrocarbons; may play a role in bone formation [RGD, Feb 2006]

Uniprot Description

AHR: a nuclear receptor for aryl hydrocarbons involved in xenobiotic metabolism, cell cycle regulation, and development in response to both endogenous and environmental signals. AhR was initially identified as a receptor for dioxins, which are environmental pollutants generated by waste incineration and other industrial processes . AhR ligands include polycyclic aromatic hydrocarbons, including the carcinogen benzo(a)pyrene and other components of cigarette smoke. Naturally occurring AhR ligands include flavonoids, which are aromatic plant secondary compounds commonly found in vegetables and fruits. Cytoplasmic aryl hydrocarbon receptors are found in protein complexes with heat shock proteins. Upon ligand binding, AhR dissociates from heat shock proteins and translocate to the nucleus where it dimerizes with AhR nuclear translocator (ARNT, HIF-1b). The AhR/ARNT heterodimer binds to nuclear xenobiotic response elements to control the expression of genes associated with xenobiotic metabolism, including several cytochrome P450 genes. AhR is ubiquitously expressed and is thought to play a role in regulation of cell proliferation and differentiation, cytokine expression, and xenobiotic metabolism. Research studies link AhR activity with the control of regulatory T-cell and T-helper 17 cell differentiation, regulation of the inflammatory response, and the onset of lung cancer.

Protein type: DNA-binding; Nuclear receptor; Transcription factor

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein dimerization activity; RNA polymerase II transcription factor activity, enhancer binding; signal transducer activity; ligand-dependent nuclear receptor activity; protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; Hsp90 protein binding; aryl hydrocarbon receptor activity; transcription factor binding; transcription factor activity

Biological Process: prostate gland development; transcription from RNA polymerase II promoter; gland development; positive regulation of cell size; blood circulation; apoptosis; response to toxin; positive regulation of transcription, DNA-dependent; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; response to organic cyclic substance; response to estradiol stimulus; smooth muscle development; ovarian follicle development; B cell homeostasis; regulation of transcription, DNA-dependent; post-embryonic hemopoiesis; regulation of B cell proliferation; T cell homeostasis; circadian regulation of gene expression; regulation of blood vessel size; positive regulation of transcriptional preinitiation complex assembly; positive regulation of growth; spleen development; blood vessel development; camera-type eye development; intracellular receptor-mediated signaling pathway; immune system process; embryonic hemopoiesis; negative regulation of systemic arterial blood pressure; liver development; negative regulation of vasoconstriction; regulation of transcription from RNA polymerase II promoter; patterning of blood vessels; response to xenobiotic stimulus; regulation of gene expression; blood vessel morphogenesis; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; reproductive structure development; lymphocyte homeostasis