Typical Testing Data/Standard Curve (for reference only)
Rat Adenosylhomocysteinase ELISA Kit | Ahcy elisa kit
Rat Adenosylhomocysteinase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
enzyme that catalyzes the hydrolysis of S-adenosylhomocysteine to form adenosine and homocysteine [RGD, Feb 2006]
Uniprot Description
SAHH: Adenosylhomocysteine is a competitive inhibitor of S- adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD). A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy. Belongs to the adenosylhomocysteinase family.
Protein type: Hydrolase; Amino Acid Metabolism - cysteine and methionine; EC 3.3.1.1; Other Amino Acids Metabolism - selenoamino acid
Cellular Component: cytoplasm; cytosol; melanosome; neuron projection; nucleus
Molecular Function: adenosylhomocysteinase activity; adenyl nucleotide binding; copper ion binding; identical protein binding; NAD binding; protein self-association
Biological Process: chronic inflammatory response to antigenic stimulus; circadian sleep/wake cycle; one-carbon compound metabolic process; response to hypoxia; response to nutrient; S-adenosylhomocysteine catabolic process; S-adenosylmethionine cycle
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Product Notes
The Rat Ahcy ahcy (Catalog #AAA2885786) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2885786 ELISA Kit recognizes Rat Ahcy. It is sometimes possible for the material contained within the vial of "Adenosylhomocysteinase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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