Related Product Information for AHA elisa kit
Principle of the Assay: Histone-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Histone antigen and an Histone-Ab-HRP conjugate. The assay sample and buffer are incubated together with Histone-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Histone-Ab concentration since Histone-Ab from samples and Histone-Ab conjugate compete for the Histone antigen binding site. Since the number of sites is limited, as more sites are occupied by Histone-Ab from the sample, fewer sites are left to bind Histone-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Histone-Ab concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AHA elisa kit
NCBI Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]
Uniprot Description
H2AZ: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP. Belongs to the histone H2A family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 4q24
Cellular Component: Barr body; nuclear heterochromatin; nucleosome; nucleus
Molecular Function: protein binding; nucleosomal DNA binding; protein heterodimerization activity; chromatin DNA binding
Biological Process: positive regulation of transcription from RNA polymerase II promoter
Product Notes
The Monkey AHA h2afz (Catalog #
AAA754919) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA754919 ELISA Kit recognizes Monkey AHA.
It is sometimes possible for the material contained within the vial of
"Anti-Histone Antibody, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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absolutely not suitable for use in any
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industry standard. AAA Biotech
and its authorized distribution partners reserve the right to refuse to fulfill any order if
we have any indication that a
purchaser may be intending to use a product outside of our accepted criteria.
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