Mouse Anti-Histone Antibody ELISA Kit | AHA elisa kit
Mouse Anti-Histone Antibody ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: HISAb ELISA kit applies the competitive enzyme immunoassay technique utilizing a HIS antigen and an HISAb-HRP conjugate. The assay sample and buffer are incubated together with HISAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HISAb concentration since HISAb from samples and HISAb-HRP conjugate compete for the HIS antigen binding site. Since the number of sites is limited, as more sites are occupied by HISAb from the sample, fewer sites are left to bind HISAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HISAb concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]
Uniprot Description
H2AZ: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP. Belongs to the histone H2A family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 4q24
Cellular Component: Barr body; nucleosome; nuclear heterochromatin; nucleus
Molecular Function: protein binding; nucleosomal DNA binding; chromatin DNA binding; protein heterodimerization activity
Biological Process: positive regulation of transcription from RNA polymerase II promoter
