Rabbit anti-Human FTO Polyclonal Antibody | anti-FTO antibody

FTO Polyclonal Antibody

Cat. Num. AAA3012723

• Gene Names: FTO; GDFD; ALKBH9; BMIQ14
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Purity: ProA affinity purified
• Synonyms: FTO; Polyclonal Antibody; FTO Polyclonal Antibody; KIAA1752; AlkB homolog 9; ALKBH9; Alpha-ketoglutarate-dependent dioxygenase FTO; AW743446; Fat mass and obesity-associated protein; FATSO; MOUSE; HOMOLOG OF; Fto; GDFD; mKIAA1752; Protein fatso; anti-FTO antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FTO polyclonal antibody detects endogenous levels of FTO protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 505

• Applicable Applications for anti-FTO antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2,000; ICC: 1:50-1:200; IF: 1:50-1:100; IHC: 1:50-1:200
• Immunogen: Recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of FTO on different lysates using anti-FTO antibody at 1/1,000 dilution. Positive control: Lane 1: Human fetal brain tissue Lane 2: HepG2 Lane 3: 293)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-FTO antibody. Counter stained with hematoxylin.)

Related Product Information for anti-FTO antibody

FTO, also known as Fatso or KIAA1752, is a 505 amino acid protein that has an N-terminal nuclear localization signal. Expressed in a variety of tissues, with highest levels present in brain and pancreatic tissue, Fatso exists as four alternatively spliced isoforms, one of which is associated with a predisposition to childhood and adult obesity. Due to its involvement in the development of obesity, Fatso is associated with an increased BMI and may be involved in the pathogenesis of type 2 diabetes. The gene encoding Fatso maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, as is Crohn's disease, which is a gastrointestinal inflammatory condition.

NCBI and Uniprot Product Information

• NCBI GI #: 122937263
• NCBI GeneID: 79068
• NCBI Accession #: NP_001073901.1
• NCBI GenBank Nucleotide #: NP_001073901.1
• UniProt Accession #: Q9C0B1
• NCBI Official Full Name: alpha-ketoglutarate-dependent dioxygenase FTO isoform 3
• NCBI Official Synonym Full Names: FTO alpha-ketoglutarate dependent dioxygenase
• NCBI Official Symbol: FTO
• NCBI Official Synonym Symbols: GDFD; ALKBH9; BMIQ14
• NCBI Protein Information: alpha-ketoglutarate-dependent dioxygenase FTO
• UniProt Protein Name: Alpha-ketoglutarate-dependent dioxygenase FTO
• Protein Family: Alpha-ketoglutarate-dependent dioxygenase
• UniProt Gene Name: FTO
• UniProt Synonym Gene Names: KIAA1752
• UniProt Entry Name: FTO_HUMAN

NCBI Description

This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]