Acylglycerol Kinase Recombinant Protein | AGK recombinant protein

Recombinant Acylglycerol Kinase (AGK)

Cat. Num. AAA2033251

• Gene Names: AGK; MULK; CATC5; CTRCT38; MTDPS10
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Acylglycerol Kinase; Recombinant Acylglycerol Kinase (AGK); MULK; Multiple Substrate Lipid Kinase; AGK recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: TTAGLC LLTWGGHWLY GKHCDNLLRR AACQEAQVFGNQLIPPNAQV KKATVFLNPA ACKGKARTLF EKNAAPILHL SGMDVTIVKTDYEGQAKKLL ELMENTDVII VAGGDGTLQE VVTGVLRRTD EATFSKIPIGFIPLGETSSL SHTLFAESGN KVQHITDATL AIVKGETVPL DVLQIKGEKEQPVFAMTGLR WGSFRDAGVK VSKYWYLGPL KIKAAHFFST LKEWPQTHQASISYTGPTER PPNEPEETPV QRPSLYRRIL RRLASYWAQP QDALSQEVSP
• Sequence Length: 422

• Applicable Applications for AGK recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 61.6kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Thr15~Pro300
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and GST-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 8922701
• NCBI GeneID: 55750
• NCBI Accession #: NP_060708.1
• NCBI GenBank Nucleotide #: NM_018238.3
• Molecular Weight: 7,634 Da
• NCBI Official Full Name: acylglycerol kinase, mitochondrial
• NCBI Official Synonym Full Names: acylglycerol kinase
• NCBI Official Symbol: AGK
• NCBI Official Synonym Symbols: MULK; CATC5; CTRCT38; MTDPS10
• NCBI Protein Information: acylglycerol kinase, mitochondrial
• UniProt Protein Name: Acylglycerol kinase, mitochondrial
• Protein Family: Acylglycerol kinase
• UniProt Gene Name: AGK
• UniProt Synonym Gene Names: MULK; hAGK; HsMuLK; MuLK; Multi-substrate lipid kinase
• UniProt Entry Name: AGK_HUMAN

NCBI Description

The protein encoded by this gene is a mitochondrial membrane protein involved in lipid and glycerolipid metabolism. The encoded protein is a lipid kinase that catalyzes the formation of phosphatidic and lysophosphatidic acids. Defects in this gene have been associated with mitochondrial DNA depletion syndrome 10. [provided by RefSeq, Feb 2012]

Uniprot Description

AGK: Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth. Defects in AGK are the cause of mitochondrial DNA depletion syndrome type 10 (MTDPS10). An autosomal recessive mitochondrial disorder characterized by congenital cataracts, hypertrophic cardiomyopathy, skeletal myopathy, exercise intolerance, and lactic acidosis. Mental development is normal, but affected individuals may die early from cardiomyopathy. Defects in AGK are the cause of cataract, congenital, autosomal recessive type 5 (CATC5). CATC5 consists of an opacification of the crystalline lens of the eye becoming evident at birth. It frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.107; Kinase, lipid; Lipid Metabolism - glycerolipid; EC 2.7.1.94; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: intracellular membrane-bound organelle; mitochondrial outer membrane; mitochondrion

Molecular Function: acylglycerol kinase activity; ATP binding; ceramide kinase activity; diacylglycerol kinase activity

Biological Process: cellular lipid metabolic process; ceramide biosynthetic process; lipid phosphorylation; triacylglycerol biosynthetic process

Disease: Cataract 38; Sengers Syndrome