Actin Filament Associated Protein 1 Recombinant Protein | AFAP1 recombinant protein

Recombinant Actin Filament Associated Protein 1 (AFAP1)

Cat. Num. AAA2033157

• Gene Names: AFAP1; AFAP; AFAP110; AFAP-110
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Actin Filament Associated Protein 1; Recombinant Actin Filament Associated Protein 1 (AFAP1); AFAP-110; 110 kDa actin filament-associated protein; AFAP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: CGYLNVLSN SRWRERWCRV KDSKLILHKD RADLKTHLVS IPLRGCEVIP GLDSKHPLTF RLLRNGQEVA VLEASSSEDM GRWIGILLAE TGSSTDPGAL HYDYIDVEMS ANVIQTAKQT FCFMNRRAVS TSPYLGSLSN GYAHPSGTAL HYDDVPCVNG SLKNKKPPAS SNGVPVKGKAPSSQQKKVET AGGVKRTASN AEQYKYGKNR VEADAKRLQS KE
• Sequence Length: 730

• Applicable Applications for AFAP1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 28.0kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Cys352~Glu572
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 40807504
• NCBI GeneID: 60312
• NCBI Accession #: NP_940997.1
• NCBI GenBank Nucleotide #: NM_198595.2
• Molecular Weight: 89,795 Da
• NCBI Official Full Name: actin filament-associated protein 1 isoform B
• NCBI Official Synonym Full Names: actin filament associated protein 1
• NCBI Official Symbol: AFAP1
• NCBI Official Synonym Symbols: AFAP; AFAP110; AFAP-110
• NCBI Protein Information: actin filament-associated protein 1
• UniProt Protein Name: Actin filament-associated protein 1
• Protein Family: Actin filament-associated protein
• UniProt Gene Name: AFAP1
• UniProt Synonym Gene Names: AFAP; AFAP-110
• UniProt Entry Name: AFAP1_HUMAN

NCBI Description

The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

AFAP: Can cross-link actin filaments into both network and bundle structures. May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: cytoplasm; cytoskeleton; focal adhesion

Molecular Function: actin binding

Biological Process: regulation of cytoskeleton organization and biogenesis; regulation of signal transduction