Rat Adrenergic Receptor Beta Kinase 2 (ADRbK2) ELISA Kit | ADRbK2 elisa kit

Rat Adrenergic Receptor Beta Kinase 2 (ADRbK2) ELISA Kit

Cat. Num. AAA2704268

• Gene Names: Grk3; Adrbk2; Bark-2; Adrbk-2; AI851927; AW551196; 4833444A01Rik
• Reactivity: Rat
• Synonyms: Adrenergic Receptor Beta Kinase 2 (ADRbK2); Rat Adrenergic Receptor Beta Kinase 2 (ADRbK2) ELISA Kit; B-ARK2; bARK2; Beta-ARK2; GRK3; G-protein-coupled receptor kinase 3; ADRbK2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adrenergic Receptor Beta Kinase 2 (ADRbK2).; No significant cross-reactivity or interference between Adrenergic Receptor Beta Kinase 2 (ADRbK2) and analogues was observed.
• Sequence Length: 688

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.78-50ng/mL
• Sensitivity: < 0.31ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenergic Receptor Beta Kinase 2 (ADRbK2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenergic Receptor Beta Kinase 2 (ADRbK2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADRbK2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adrenergic Receptor Beta Kinase 2 (ADRbK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adrenergic Receptor Beta Kinase 2 (ADRbK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adrenergic Receptor Beta Kinase 2 (ADRbK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adrenergic Receptor Beta Kinase 2 (ADRbK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 157170074
• NCBI GeneID: 320129
• Molecular Weight: 79,657 Da
• NCBI Official Full Name: Adrenergic receptor kinase, beta 2
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 3
• NCBI Official Symbol: Grk3
• NCBI Official Synonym Symbols: Adrbk2; Bark-2; Adrbk-2; AI851927; AW551196; 4833444A01Rik
• NCBI Protein Information: beta-adrenergic receptor kinase 2
• UniProt Protein Name: Beta-adrenergic receptor kinase 2
• Protein Family: Beta-adrenergic receptor kinase
• UniProt Gene Name: Adrbk2
• UniProt Synonym Gene Names: Beta-ARK-2

Uniprot Description

GRK3: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Protein type: AGC group; BARK subfamily; EC 2.7.11.15; GRK family; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 5 F|5 55.29 cM

Cellular Component: axon; cilium; cytosol; dendrite terminus; dendritic shaft; dendritic spine; membrane; nucleus; perinuclear region of cytoplasm; synapse; Z disc

Molecular Function: ATP binding; beta-adrenergic receptor kinase activity; D1 dopamine receptor binding; G-protein coupled receptor kinase activity; kinase activity; nucleotide binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity

Biological Process: desensitization of G-protein coupled receptor protein signaling pathway; inositol phosphate metabolic process; intracellular protein transport; phosphorylation; positive regulation of protein phosphorylation; protein amino acid phosphorylation; receptor internalization; rhodopsin metabolic process; signal transduction