ADPRH recombinant protein

ADPRH Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121862

• Gene Names: ADPRH; ARH1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: ADPRH; ADPRH Protein; Human; Recombinant (His Tag); Human ADPRH/ARH1 Protein (His Tag); ARH1 Protein; ADP-ribosylarginine hydrolase; ADPRH recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu351

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human ADPRH (NP_001116.1)(Met1-Leu351) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ADPRH recombinant protein

Background: Cholera toxin (CT) produced by Vibrio cholerae causes the devastating diarrhea of cholera by catalyzing the ADP-ribosylation of the alpha subunit of the intestinal Gs protein (Gsalpha), leading to characteristic water and electrolyte losses. Mammalian cells contain ADP-ribosyltransferases similar to CT and an ADP-ribosyl(arginine)protein hydrolase (ADPRH), which cleaves the ADP-ribose-(arginine)protein bond, regenerating native protein and completing an ADP-ribosylation cycle. CT-catalyzed ADP-ribosylation of cell proteins can be counteracted by ADPRH, which could function as a modifier gene in disease. Further, our study demonstrates that enzymatic cross talk exists between bacterial toxin ADP-ribosyltransferases and host ADP-ribosylation cycles. In disease, toxin-catalyzed ADP-ribosylation overwhelms this potential host defense system, resulting in persistence of ADP-ribosylation and intoxication of the cell. Mono-ADP-ribosylation is a reversible modification of proteins with NAD:arginine ADP-ribosyltransferases and ADP-ribosylarginine hydrolases (ADPRH) catalyzing the opposing arms of an ADP-ribosylation cycle. The ADPRH cDNA had been cloned from human, rat, and mouse tissues and high levels of mRNA were found in brain, spleen, and testis. Human ADP-ribosylhydrolase 1 (hARH1, ADPRH) cleaves the glycosidic bond of ADP-ribose attached to an Arg residue of a protein.

NCBI and Uniprot Product Information

• NCBI GI #: 4557263
• NCBI GeneID: 141
• NCBI Accession #: NP_001116.1
• NCBI GenBank Nucleotide #: NM_001125.3
• UniProt Accession #: P54922; B2R8H1; D3DN83
• Molecular Weight: 39,507 Da
• NCBI Official Full Name: ADP-ribosylarginine hydrolase isoform 1
• NCBI Official Synonym Full Names: ADP-ribosylarginine hydrolase
• NCBI Official Symbol: ADPRH
• NCBI Official Synonym Symbols: ARH1
• NCBI Protein Information: ADP-ribosylarginine hydrolase; ADP-ribose-L-arginine cleaving enzyme
• UniProt Protein Name: [Protein ADP-ribosylarginine] hydrolase
• Protein Family: [Protein ADP-ribosylarginine] hydrolase
• UniProt Gene Name: ADPRH
• UniProt Synonym Gene Names: ARH1; ADP-ribosylarginine hydrolase
• UniProt Entry Name: ADPRH_HUMAN

NCBI Description

The enzyme encoded by this gene catalyzes removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. Unlike the rat and mouse enzymes that require DTT for maximal activity, the human enzyme is DTT-independent. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, May 2014]

Uniprot Description

ADPRH: Catalyzes the reverse reaction of mono-ADP-ribosylation. Belongs to the ADP-ribosylglycohydrolase family.

Protein type: EC 3.2.2.19; Hydrolase

Chromosomal Location of Human Ortholog: 3q13.31-q13.33

Molecular Function: ADP-ribosylarginine hydrolase activity; magnesium ion binding

Biological Process: protein amino acid de-ADP-ribosylation; protein modification process