Rabbit ACK1 Polyclonal Antibody | anti-ACK1 antibody

Phospho-ACK1 (Tyr857+Tyr858)[Tyr859+Tyr860] Antibody

Cat. Num. AAA9614339

• Gene Names: TNK2; ACK; ACK1; ACK-1; p21cdc42Hs
• Reactivity: Human, Mouse; Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (91%), Dog (100%), Chicken (100%), Xenopus (91%)
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: ACK1; Polyclonal Antibody; Phospho-ACK1 (Tyr857+Tyr858)[Tyr859+Tyr860] Antibody; Acetate kinase 1; Acetokinase 1; ACK 1; ACK; ACK-1; ACK1_HUMAN; Activated Cdc42 associated kinase 1; Activated CDC42 kinase 1; Activated p21cdc42Hs kinase; FLJ44758; FLJ45547; p21cdc42Hs; TNK 2; TNK2; Tyrosine kinase non receptor 2; Tyrosine kinase non receptor protein 2; Tyrosine kinase non-receptor protein 2; anti-ACK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (91%), Dog (100%), Chicken (100%), Xenopus (91%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-ACK1 (Tyr857+Tyr858) Antibody detects endogenous levels of ACK1 only when phosphorylated at Tyr859+Tyr860, which site historically referenced as Tyr857+Tyr858.; Tissue Specificity: The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lympHnode metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ACK1 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human ACK1 around the phosphorylation site of Tyr857 and Tyr858.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC and is observed during association with clathrin-coated pits.Polyubiquitinated by NEDD4 and NEDD4L. Degradation can be induced by EGF and is lysosome-dependent (By similarity).
• Subunit Structure: Interacts with NEDD4 (via WW3 domain). NEDD4L and EGF promote association with NEDD4 (By similarity). Homodimer. Interacts with AR, CDC42, WWASL and WWOX. Interacts with CSPG4 (activated). Interacts with MERTK (activated); stimulates autophosphorylation. May interact (phosphorylated) with HSP90AB1; maintains kinase activity. Interacts with NPHP1. Interacts with SNX9 (via SH3 domain). Interacts with SRC (via SH2 and SH3 domain). Interacts with EGFR, and this interaction is dependent on EGF stimulation and kinase activity of EGFR. Interacts (via kinase domain) with AKT1. Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas. Interacts with BCAR1/p130cas via SH3 domains. Forms complexes with GRB2 and numerous receptor tyrosine kinases (RTK) including LTK, AXL or PDGFRL, in which GRB2 promotes RTK recruitment by TNK2.
• Similarity: The EBD (EGFR-binding domain) domain is necessary for interaction with EGFR.The SAM-like domain is necessary for NEDD4-mediated ubiquitination. Promotes membrane localization and dimerization to allow for autophosphorylation.The UBA domain binds both poly- and mono-ubiquitin.Belongs to the protein kinase superfamily. Tyr protein kinase family.
• Subcellular Location: Cell membrane. Nucleus. Endosome. Cell junction>Adherens junction. Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle>Clathrin-coated vesicle. Membrane>Clathrin-coated pit. Cytoplasm>Perinuclear region.; Note: The Tyr-284 phosphorylated form is found both in the membrane and nucleus. Colocalizes with EGFR on endosomes. Nuclear translocation is CDC42-dependent.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Mouse heart tissue, using Phospho-Ack1 (Tyr857+Tyr858) Antibody.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-ACK1 antibody

Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP.

NCBI and Uniprot Product Information

• NCBI GI #: 58331191
• NCBI GeneID: 10188
• NCBI Accession #: NP_001010938.1
• NCBI GenBank Nucleotide #: NM_001010938.1
• UniProt Accession #: Q07912; Q6ZMQ0; Q8N6U7; Q96H59
• Molecular Weight: 114,569 Da
• NCBI Official Full Name: activated CDC42 kinase 1 isoform 2
• NCBI Official Synonym Full Names: tyrosine kinase, non-receptor, 2
• NCBI Official Symbol: TNK2
• NCBI Official Synonym Symbols: ACK; ACK1; ACK-1; p21cdc42Hs
• NCBI Protein Information: activated CDC42 kinase 1; activated p21cdc42Hs kinase; activated Cdc42-associated kinase 1; tyrosine kinase non-receptor protein 2
• UniProt Protein Name: Activated CDC42 kinase 1
• Protein Family: Activator of C kinase protein
• UniProt Gene Name: TNK2
• UniProt Synonym Gene Names: ACK1; ACK-1
• UniProt Entry Name: ACK1_HUMAN

NCBI Description

This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Ack: a tyrosine kinase that binds Cdc42 in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. May be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42 and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Amplification in primary tumors correlates with metastatic potential . Two differentially spliced isoforms have been described.

Protein type: Protein kinase, TK; EC 2.7.11.1; Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Ack family

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: extrinsic to internal side of plasma membrane; adherens junction; growth cone; cytoplasmic vesicle membrane; cell soma; membrane; clathrin-coated vesicle; axon; cytoplasm; dendrite; plasma membrane; coated pit; nucleus; endosome

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; WW domain binding; ATP binding; GTPase inhibitor activity; hormone receptor binding; epidermal growth factor receptor binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; cell migration; cell surface receptor linked signal transduction; small GTPase mediated signal transduction; negative regulation of catalytic activity; innate immune response; endocytosis; cell differentiation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation