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Alcohol Dehydrogenase 3 Recombinant Protein | ADH3 recombinant protein

Recombinant Alcohol Dehydrogenase 3 (ADH3)

Gene Names
ADH1C; ADH3
Applications
SDS-Page, Western Blot
Purity
>90%
Synonyms
Alcohol Dehydrogenase 3; Recombinant Alcohol Dehydrogenase 3 (ADH3); ADH1C; Alcohol dehydrogenase subunit gamma; ADH3 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
>90%
Form/Format
Freeze-dried powder
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence Positions
Ser2~Phe375
Sequence Length
375
Applicable Applications for ADH3 recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Source
Prokaryotic expression
Species
Human
Tag
N-terminal His and GST Tag
Subcellular Location
Secreted
Isoelectric Point
8.6
Reconstitution
Reconstitute in PBS or others.
Preparation and Storage
Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Product Categories/Family for ADH3 recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
126
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 69.7kDa
Accurate Molecular Mass: 70kDa
NCBI Official Full Name
alcohol dehydrogenase 1C
NCBI Official Synonym Full Names
alcohol dehydrogenase 1C (class I), gamma polypeptide
NCBI Official Symbol
ADH1C
NCBI Official Synonym Symbols
ADH3
NCBI Protein Information
alcohol dehydrogenase 1C
UniProt Protein Name
Alcohol dehydrogenase 1C
Protein Family
UniProt Gene Name
ADH1C
UniProt Synonym Gene Names
ADH3
UniProt Entry Name
ADH1G_HUMAN

NCBI Description

This gene encodes class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation to acetaldehyde, thus playing a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. An association between ADH1C polymorphism and alcohol dependence has not been established. [provided by RefSeq, Sep 2019]

Uniprot Description

ADH1C: class I alcohol dehydrogenase, gamma subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. [provided by RefSeq, Jul 2008]

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Amino Acid Metabolism - tyrosine; Oxidoreductase; EC 1.1.1.1; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lipid Metabolism - fatty acid; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol

Molecular Function: zinc ion binding; alcohol dehydrogenase activity

Biological Process: xenobiotic metabolic process; ethanol oxidation

Disease: Alcohol Dependence; Parkinson Disease, Late-onset

Research Articles on ADH3

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Product Notes

The ADH3 adh1c (Catalog #AAA2123338) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Ser2~Phe375. AAA Biotech's Alcohol Dehydrogenase 3 can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the ADH3 adh1c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alcohol Dehydrogenase 3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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