Human adipose differentiation-related protein ELISA Kit | ADFP elisa kit

Human adipose differentiation-related protein, ADRP ELISA Kit

Cat. Num. AAA704996

• Gene Names: PLIN2; ADFP; ADRP
• Reactivity: Human
• Synonyms: adipose differentiation-related protein; Human adipose differentiation-related protein; ADRP ELISA Kit; RP11-151J10.1; ADFP; ADRP; MGC10598; adipophilin; ADFP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ADFP. No significant cross-reactivity or interference between Human ADFP and analogues was observed.
• Sequence Length: 437

• Samples: Serum, urine, cell culture supernates, tissue homogenates
• Detection Range: 3.12 ng/ml-200 ng/ml
• Sensitivity: 0.78 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADFP elisa kit

Introduction: Adipose differentiation-related protein, is a protein which in humans is encoded by the ADRP gene. Adipocyte differentiation-related protein is associated with the globule surface membrane material. This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADRP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ADRP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 34577059
• NCBI GeneID: 123
• NCBI Accession #: NP_001113.2
• NCBI GenBank Nucleotide #: NM_001122.3
• UniProt Accession #: Q99541; Q9BSC3
• Molecular Weight: 48,075 Da
• NCBI Official Full Name: perilipin-2
• NCBI Official Synonym Full Names: perilipin 2
• NCBI Official Symbol: PLIN2
• NCBI Official Synonym Symbols: ADFP; ADRP
• NCBI Protein Information: perilipin-2; adipophilin; adipose differentiation-related protein
• UniProt Protein Name: Perilipin-2
• UniProt Gene Name: PLIN2
• UniProt Synonym Gene Names: ADFP; ADRP
• UniProt Entry Name: PLIN2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

Function: May be involved in development and maintenance of adipose tissue