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Typical Testing Data

Human Pituitary adenylate cyclase-activating polypeptide type I receptor ELISA Kit | ADCYAP1R1 elisa kit

Human Pituitary adenylate cyclase-activating polypeptide type I receptor ELISA Kit

Gene Names
Adcyap1r1; PAC1-R; PACAPR1; PACAP-R1; PACAPR1A; PACAP-R1A
Reactivity
Human
Synonyms
Pituitary adenylate cyclase-activating polypeptide type I receptor; Human Pituitary adenylate cyclase-activating polypeptide type I receptor ELISA Kit; ADCYAP1R1/Pituitary adenylate cyclase-activating polypeptide type I receptor/PACAP type I receptor/PACAP-R-1/PACAP-R1/PAC1/PAC1R/PACAPR/PACAPRI; ADCYAP1R1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
523
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Testing Data

Typical Standard Curve

Typical Standard Curve
Related Product Information for ADCYAP1R1 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ADCYAP1R1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ADCYAP1R1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ADCYAP1R1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ADCYAP1R1 can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,233 Da
NCBI Official Full Name
pituitary adenylate cyclase-activating polypeptide type I receptor isoform a
NCBI Official Synonym Full Names
adenylate cyclase activating polypeptide 1 receptor type 1
NCBI Official Symbol
Adcyap1r1
NCBI Official Synonym Symbols
PAC1-R; PACAPR1; PACAP-R1; PACAPR1A; PACAP-R1A
NCBI Protein Information
pituitary adenylate cyclase-activating polypeptide type I receptor
UniProt Protein Name
Pituitary adenylate cyclase-activating polypeptide type I receptor
UniProt Gene Name
Adcyap1r1
UniProt Synonym Gene Names
PACAP type I receptor; PACAP-R-1; PACAP-R1
UniProt Entry Name
PACR_RAT

NCBI Description

regulates neural precursor proliferation; mediates inhibitory signaling for Shh-induced cerebellar granule precursor cell proliferation [RGD, Feb 2006]

Uniprot Description

ADCYAP1R1: This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract. Belongs to the G-protein coupled receptor 2 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 2

Cellular Component: caveola; cell surface; cytoplasm; endosome; Golgi membrane; integral to membrane; neuron projection; receptor complex; rough endoplasmic reticulum; tight junction

Molecular Function: adenylate cyclase binding; ADP-ribosylation factor binding; neuropeptide binding; vasoactive intestinal polypeptide receptor activity

Biological Process: cAMP-mediated signaling; cell differentiation; cell surface receptor linked signal transduction; development of primary female sexual characteristics; G-protein coupled receptor protein signaling pathway; multicellular organismal response to stress; phospholipase C activation; positive regulation of cAMP biosynthetic process; response to drug; response to estradiol stimulus; response to ethanol; spermatogenesis

Research Articles on ADCYAP1R1

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Product Notes

The Human ADCYAP1R1 adcyap1r1 (Catalog #AAA762897) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA762897 ELISA Kit recognizes Human ADCYAP1R1. It is sometimes possible for the material contained within the vial of "Pituitary adenylate cyclase-activating polypeptide type I receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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