Bovine Adenylate Cyclase Activating Polypeptide 1, Pituitary ELISA Kit | ADCYAP1 elisa kit

Bovine Adenylate Cyclase Activating Polypeptide 1, Pituitary ELISA Kit

Cat. Num. AAA741682

• Gene Names: ADCYAP1; PACAP
• Reactivity: Bovine
• Synonyms: Adenylate Cyclase Activating Polypeptide 1; Pituitary; Bovine Adenylate Cyclase Activating Polypeptide 1; Pituitary ELISA Kit; ADCYAP1 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/Ml
• Intended Uses: This ADCYAP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ADCYAP1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADCYAP1 elisa kit

Principle of the Assay: ADCYAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADCYAP1 antibody and an ADCYAP1-HRP conjugate. The assay sample and buffer are incubated together with ADCYAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADCYAP1 concentration since ADCYAP1 from samples and ADCYAP1-HRP conjugate compete for the anti-ADCYAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADCYAP1 from the sample, fewer sites are left to bind ADCYAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADCYAP1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ADCYAP1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119622111
• NCBI GeneID: 116
• Molecular Weight: 18,835 Da
• NCBI Official Full Name: adenylate cyclase activating polypeptide 1 (pituitary)
• NCBI Official Synonym Full Names: adenylate cyclase activating polypeptide 1 (pituitary)
• NCBI Official Symbol: ADCYAP1
• NCBI Official Synonym Symbols: PACAP
• NCBI Protein Information: pituitary adenylate cyclase-activating polypeptide; prepro-PACAP
• UniProt Protein Name: Pituitary adenylate cyclase-activating polypeptide
• Protein Family: Glucagon family neuropeptides
• UniProt Gene Name: ADCYAP1
• UniProt Synonym Gene Names: PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38
• UniProt Entry Name: PACA_HUMAN

NCBI Description

This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]

Uniprot Description

ADCYAP1: Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Belongs to the glucagon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18p11

Cellular Component: extracellular space; extracellular region; terminal button

Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; receptor signaling protein activity; pituitary adenylate cyclase-activating peptide receptor binding; pituitary adenylate cyclase activating polypeptide activity; receptor binding

Biological Process: behavioral fear response; nerve growth factor receptor signaling pathway; negative regulation of potassium ion transport; female pregnancy; sensory perception of pain; positive regulation of vasodilation; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell cycle; histamine secretion; cAMP-mediated signaling; elevation of cytosolic calcium ion concentration; regulation of protein localization; ovarian follicle development; cell-cell signaling; neuropeptide signaling pathway; positive regulation of cell proliferation; neurite development; adenylate cyclase activation; negative regulation of acute inflammatory response to antigenic stimulus; positive regulation of interleukin-6 production; response to starvation; positive regulation of growth hormone secretion; ATP metabolic process; response to ethanol; regulation of postsynaptic membrane potential; pituitary gland development; positive regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of acute inflammatory response to non-antigenic stimulus; regulation of G-protein coupled receptor protein signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway