Mouse SRC1 (Steroid receptor coactivator 1) ELISA Kit | SRC1 elisa kit

Mouse SRC1 (Steroid receptor coactivator 1) ELISA Kit

Cat. Num. AAA7606862

• Gene Names: NCOA1; SRC1; KAT13A; RIP160; F-SRC-1; bHLHe42; bHLHe74
• Reactivity: Mouse
• Synonyms: SRC1 (Steroid receptor coactivator 1); Mouse SRC1 (Steroid receptor coactivator 1) ELISA Kit; SRC1 elisa kit

• Reactivity: Mouse
• Sequence Length: 1441

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SRC1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 1906028
• NCBI GeneID: 8648
• Molecular Weight: 156,628 Da
• NCBI Official Full Name: steroid receptor coactivator-1
• NCBI Official Synonym Full Names: nuclear receptor coactivator 1
• NCBI Official Symbol: NCOA1
• NCBI Official Synonym Symbols: SRC1; KAT13A; RIP160; F-SRC-1; bHLHe42; bHLHe74
• NCBI Protein Information: nuclear receptor coactivator 1
• UniProt Protein Name: Nuclear receptor coactivator 1
• Protein Family: Protein
• UniProt Gene Name: NCOA1
• UniProt Synonym Gene Names: BHLHE74; SRC1; NCoA-1; bHLHe74; SRC-1

NCBI Description

The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

SRC-1: a transcriptional coactivator for steroid and nuclear hormone receptors. A member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, and bHLH and PAS domains. Phosphorylated and activated via the mitogen-activated protein kinase pathway. Three alternatively spliced isoforms have been described.

Protein type: Acetyltransferase; DNA-binding; EC 2.3.1.48; Nuclear receptor co-regulator; Oncoprotein; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: cytosol; nuclear chromatin; nucleoplasm; plasma membrane; protein complex

Molecular Function: aryl hydrocarbon receptor binding; enzyme binding; estrogen receptor binding; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding; protein binding; protein N-terminus binding; transcription coactivator activity

Biological Process: bile acid and bile salt transport; cellular lipid metabolic process; cellular response to hormone stimulus; intracellular receptor-mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter by galactose; positive regulation of transcription, DNA-dependent; transcription, DNA-dependent