Rabbit anti-Mouse TLE2 Polyclonal Antibody | anti-TLE2 antibody

TLE2, CT (TLE2, Transducin-like enhancer protein 2, Enhancer of split groucho-like protein 2) (Azide free) (HRP)

Cat. Num. AAA6355793

• Gene Names: TLE2; ESG; ESG2; GRG2
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TLE2; Polyclonal Antibody; CT (TLE2; Transducin-like enhancer protein 2; Enhancer of split groucho-like protein 2) (Azide free) (HRP); Enhancer of split groucho-like protein 2; anti-TLE2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 743

• Applicable Applications for anti-TLE2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TLE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636-665 amino acids from the C-terminal region of human TLE2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TLE2 Antibody (C-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the TLE2 antibody detected the TLE2 protein (arrow).)

Related Product Information for anti-TLE2 antibody

TLE2 is a transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).

Product Categories/Family for anti-TLE2 antibody

Antibodies; Transcription Factors

References

He, Z., et al. J. Virol. 84(4):2047-2062(2010). Arce, L., et al. BMC Cancer 9, 159 (2009): Higa, L.A., et al. Nat. Cell Biol. 8(11):1277-1283(2006). Javed, A., et al. J. Cell. Sci. 113 (PT 12), 2221-2231 (2000): Grbavec, D., et al. Biochem. J. 337 (PT 1), 13-17 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 21361151
• NCBI GeneID: 7089
• NCBI Accession #: NP_003251.2; NP_001138234.1; NP_001138233.1
• NCBI GenBank Nucleotide #: NP_003251.2
• UniProt Accession #: Q04725
• NCBI Official Full Name: transducin-like enhancer protein 2 isoform 1
• NCBI Official Synonym Full Names: TLE family member 2, transcriptional corepressor
• NCBI Official Symbol: TLE2
• NCBI Official Synonym Symbols: ESG; ESG2; GRG2
• NCBI Protein Information: transducin-like enhancer protein 2
• UniProt Protein Name: Transducin-like enhancer protein 2
• Protein Family: Transducin-like enhancer protein
• UniProt Gene Name: TLE2
• UniProt Synonym Gene Names: ESG2
• UniProt Entry Name: TLE2_HUMAN

Uniprot Description

TLE2: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Homooligomer and heterooligomer with other family members. Binds LEF1, TCF7, TCF7L1, TCF7L2, UTY, HES1 and HES5. In all tissues examined, mostly in heart, brain, and muscle. Belongs to the WD repeat Groucho/TLE family.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; extracellular space; nucleus

Molecular Function: protein binding; transcription corepressor activity

Biological Process: Notch signaling pathway; organ morphogenesis; Wnt receptor signaling pathway; transcription, DNA-dependent; negative regulation of transcription, DNA-dependent; signal transduction