Human A disintegrin and metalloproteinase with thrombospondin motifs 8 ELISA Kit | ADAMTS8 elisa kit

Human A disintegrin and metalloproteinase with thrombospondin motifs 8 ELISA Kit

Cat. Num. AAA763273

• Gene Names: ADAMTS8; METH2; ADAM-TS8
• Reactivity: Human
• Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 8; Human A disintegrin and metalloproteinase with thrombospondin motifs 8 ELISA Kit; ADAMTS8/A disintegrin and metalloproteinase with thrombospondin motifs 8/ADAM-TS 8/ADAM-TS8/ADAMTS-8/METH-2/METH-8/METH2/ADAM metallopeptidase with thrombospondin type 1 motif; 8/ADAM-TS8A disintegrin and metalloproteinase with thrombospondin motifs 8; ADAMTS8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ADAMTS8. No significant cross-reactivity or interference between ADAMTS8 and analogues was observed.
• Sequence Length: 889

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAMTS8 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 153792351
• NCBI GeneID: 11095
• NCBI Accession #: NP_008968.4
• NCBI GenBank Nucleotide #: NM_007037.4
• Molecular Weight: 96,460 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 8 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif 8
• NCBI Official Symbol: ADAMTS8
• NCBI Official Synonym Symbols: METH2; ADAM-TS8
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 8
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 8
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS8
• UniProt Synonym Gene Names: METH2; ADAM-TS 8; ADAM-TS8; ADAMTS-8
• UniProt Entry Name: ATS8_HUMAN

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs, and disrupts angiogenesis in vivo. A number of disorders have been mapped in the vicinity of this gene, most notably lung neoplasms. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAMTS8: Has anti-angiogenic properties.

Protein type: Cell cycle regulation; Secreted; EC 3.4.24.-; Motility/polarity/chemotaxis; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 11q25

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: heparin binding; integrin binding; low affinity phosphate transmembrane transporter activity; metalloendopeptidase activity; metallopeptidase activity; zinc ion binding

Biological Process: negative regulation of cell proliferation; proteolysis