Mouse anti-Human DCN / Decorin Monoclonal Antibody | anti-DCN antibody

Mouse Monoclonal [clone 3H4-1F4] (IgG2b,k) to Human DCN / Decorin

Cat. Num. AAA246308

• Gene Names: DCN; CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: DCN / Decorin; Monoclonal Antibody; Mouse Monoclonal [clone 3H4-1F4] (IgG2b; k) to Human DCN / Decorin; Anti-DCN / Decorin Antibody (clone 3H4-1F4) IHC-plus; DCN; CSCD; Decorin proteoglycan; PG-S2; PG40; Proteoglycan core protein; SLRR1B; Small leucine-rich protein 1B; PGII; PGS2; Bone proteoglycan II; Decorin; DSPG2; Human DCN; anti-DCN antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 3H4-1F4
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4. Sourced in Ascites.
• Concentration: 0.47 mg/ml (varies by lot)
• Sequence Length: 359

• Applicable Applications for anti-DCN antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB
• Target Species: Human
• Immunogen Description: DCN (AAH05322, 1 a.a. ~ 360 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: DCN / Decorin antibody was raised against dCN (AAH05322, 1 a.a. ~ 360 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-DCN / Decorin antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-DCN / Decorin antibody IHC of human soft tissue. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of DCN expression in transfected 293T cell line. Lane 1: DCN transfected lysate (Predicted MW: 39.7 KDa). Lane 2: Non-transfected lysate.)

ELISA (EIA)

(Detection limit for recombinant GST tagged DCN is approximately 1 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4503271
• NCBI GeneID: 1634
• NCBI Accession #: NP_001911.1
• NCBI GenBank Nucleotide #: NM_001920.3
• UniProt Accession #: P07585; Q9P0Z0; Q9P0Z1; Q9Y5N8; Q9Y5N9
• Molecular Weight: 8,266 Da
• NCBI Official Full Name: decorin isoform a preproprotein
• NCBI Official Synonym Full Names: decorin
• NCBI Official Symbol: DCN
• NCBI Official Synonym Symbols: CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
• NCBI Protein Information: decorin; PG-S2; bone proteoglycan II; decorin proteoglycan; proteoglycan core protein; small leucine-rich protein 1B; dermatan sulphate proteoglycans II
• UniProt Protein Name: Decorin
• Protein Family: Decorin
• UniProt Gene Name: DCN
• UniProt Synonym Gene Names: SLRR1B
• UniProt Entry Name: PGS2_HUMAN

NCBI Description

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

DCN: May affect the rate of fibrils formation. Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD). Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Extracellular matrix

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: extracellular matrix; lysosomal lumen; proteinaceous extracellular matrix; extracellular space; Golgi lumen; extracellular region; collagen type VI

Molecular Function: collagen binding; glycosaminoglycan binding; extracellular matrix binding; protein N-terminus binding

Biological Process: extracellular matrix organization and biogenesis; skeletal muscle development; chondroitin sulfate biosynthetic process; wound healing; glycosaminoglycan metabolic process; pathogenesis; response to lipopolysaccharide; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; extracellular matrix disassembly; organ morphogenesis; response to mechanical stimulus; chondroitin sulfate catabolic process; carbohydrate metabolic process; kidney development; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; placenta development; aging

Disease: Corneal Dystrophy, Congenital Stromal