Human ADAM metallopeptidase domain 12 ELISA Kit | ADAM12 elisa kit

Human A Disintegrin And Metalloprotease 12, ADAM12 ELISA Kit

Cat. Num. AAA704578

• Gene Names: ADAM12; MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1
• Reactivity: Human
• Synonyms: ADAM metallopeptidase domain 12; Human A Disintegrin And Metalloprotease 12; ADAM12 ELISA Kit; RP11-295J3.5; MCMP; MCMPMltna; MLTN; MLTNA; a disintegrin and metalloproteinase domain 12 (meltrin alpha) ; meltrin alpha; ADAM12 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse ACV-B. No significant cross-reactivity or interference between mouse ACV-B and analogues was observed.
• Sequence Length: 737

• Samples: Serum, Plasma And Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 6.25 pg/ml-400 pg/ml
• Sensitivity: 1.56 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM12 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACV-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACV-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACV-B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACV-B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 572882491
• NCBI GeneID: 8038
• NCBI Accession #: NP_001275903.1
• NCBI GenBank Nucleotide #: NM_001288974.1
• UniProt Accession #: O43184; O60470; Q5JRP0; Q5JRP1; Q6P9E3; Q6UWB0
• Molecular Weight: 99,542 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 12 isoform 4 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 12
• NCBI Official Symbol: ADAM12
• NCBI Official Synonym Symbols: MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 12; meltrin-alpha; metalloprotease-disintegrin 12 transmembrane
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 12
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM12
• UniProt Synonym Gene Names: MLTN; UNQ346/PRO545; ADAM 12
• UniProt Entry Name: ADA12_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]

Uniprot Description

Function: Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors

Cofactor: Binds 1 zinc ion per subunit.