ACVR1 recombinant protein

Recombinant Human ACVR1 protein

Cat. Num. AAA1562749

• Gene Names: ACVR1; FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: ACVR1; Recombinant Human ACVR1 protein; Activin receptor type-1; Activin receptor type I; ACTR-I; Activin receptor-like kinase 2; ALK-2; Serine/threonine-protein kinase receptor R1; SKR1; TGF-B superfamily receptor type I; TSR-I; ACVR1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human ACVR1 (Ile208-Lys340) was fused with GST tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for ACVR1 recombinant protein

ALK-2, also termed as ACVR1, was initially identified as an activin type I receptor because of its ability to bind activin in concert with ActRII or ActRIIB. ALK-2 is also identified as a BMP type I receptor. It has been demonstrated that ALK-2 forms complex with either the BMP-2/7-bound BMPR-II or ACVR2A /ACVR2B. ALK-1 and ALK-2 presenting in the yeast Saccharomyces cerevisiae are two haspin homologues. Both ALK-1 and ALK-2 exhibit a weak auto-kinase activity in vitro, and are phosphoproteins in vivo. ALK-1 and ALK-2 levels peak in mitosis and late-S/G2. Control of protein stability plays a major role in ALK-2 regulation. The half-life of ALK-2 is particularly short in G1. Overexpression of ALK-2, but not of ALK-1, causes a mitotic arrest, which is correlated to the kinase activity of the protein. This suggests a role for ALK-2 in the control of mitosis. Endoglin is phosphorylated on cytosolic domain threonine residues by the TGF-beta type I receptors ALK-2 and ALK-5 in prostate cancer cells. Endoglin did not inhibit cell migration in the presence of constitutively active ALK-2. Defects in ALK-2 are a cause of fibrodysplasia ossificans progressiva (FOP).

Product Categories/Family for ACVR1 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 4501895
• NCBI GeneID: 90
• NCBI Accession #: NP_001096
• NCBI GenBank Nucleotide #: NP_001096.1
• UniProt Accession #: Q04771
• NCBI Official Full Name: activin receptor type-1
• NCBI Official Synonym Full Names: activin A receptor type 1
• NCBI Official Symbol: ACVR1
• NCBI Official Synonym Symbols: FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2
• NCBI Protein Information: activin receptor type-1
• UniProt Protein Name: Activin receptor type-1
• Protein Family: Activin receptor
• UniProt Gene Name: ACVR1
• UniProt Synonym Gene Names: ACVRLK2; ACTR-I; ALK-2; SKR1; TSR-I
• UniProt Entry Name: ACVR1_HUMAN

NCBI Description

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. This gene encodes activin A type I receptor which signals a particular transcriptional response in concert with activin type II receptors. Mutations in this gene are associated with fibrodysplasia ossificans progressive. [provided by RefSeq, Jul 2008]

Uniprot Description

ALK2: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin. May be involved for left-right pattern formation during embryogenesis. Interacts with FKBP1A. Interacts with FCHO1. Expressed in normal parenchymal cells, endothelial cells, fibroblasts and tumor-derived epithelial cells. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

Protein type: Membrane protein, integral; Kinase, protein; EC 2.7.11.30; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; TKL group; STKR family; Type1 subfamily

Chromosomal Location of Human Ortholog: 2q23-q24

Cellular Component: apical part of cell; integral to plasma membrane; activin receptor complex

Molecular Function: protein homodimerization activity; metal ion binding; peptide hormone binding; transmembrane receptor protein serine/threonine kinase activity; protein kinase activity; protein serine/threonine kinase activity; protein binding; activin receptor activity, type I; transforming growth factor beta receptor activity, type I; transforming growth factor beta binding; activin binding; SMAD binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: pharyngeal system development; in utero embryonic development; positive regulation of transcription, DNA-dependent; peptidyl-threonine phosphorylation; activin receptor signaling pathway; gastrulation with mouth forming second; positive regulation of bone mineralization; protein amino acid phosphorylation; germ cell development; negative regulation of signal transduction; BMP signaling pathway; patterning of blood vessels; positive regulation of osteoblast differentiation; negative regulation of activin receptor signaling pathway; mesoderm formation; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; smooth muscle cell differentiation; regulation of skeletal muscle development; determination of left/right symmetry; regulation of ossification; neural crest cell migration; acute inflammatory response; urogenital system development; G1/S transition of mitotic cell cycle

Disease: Fibrodysplasia Ossificans Progressiva