Rabbit anti-Human ATP5J2 Polyclonal Antibody | anti-ATP5J2 antibody

ATP5J2, ID (ATP5J2, ATP5JL, ATP synthase subunit f, mitochondrial) (FITC)

Cat. Num. AAA6276582

• Gene Names: ATP5J2; ATP5JL
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATP5J2; Polyclonal Antibody; ID (ATP5J2; ATP5JL; ATP synthase subunit f; mitochondrial) (FITC); mitochondrial; anti-ATP5J2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ATP5J2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ATP5J2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-56 amino acids from the Central region of human ATP5J2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ATP5J2 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ATP5J2 antibody detected the ATP5J2 protein (arrow).)

Immunohistochemistry (IHC)

(ATP5J2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP5J2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-ATP5J2 antibody

Antibodies; Mitochondrial Proteins

References

Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008). Stelzl, U., et al. Cell 122(6):957-968(2005). Cross, R.L. Nature 427(6973):407-408(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4757812
• NCBI GeneID: 9551
• NCBI Accession #: NP_004880.1
• NCBI GenBank Nucleotide #: NM_004889.3
• UniProt Accession #: P56134; O76079; Q6IBB3; Q96L83; Q9BTI8; C9J8H9; F8W7V3
• Molecular Weight: 5,741 Da
• NCBI Official Full Name: ATP synthase subunit f, mitochondrial isoform 2a
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial Fo complex subunit F2
• NCBI Official Symbol: ATP5J2
• NCBI Official Synonym Symbols: ATP5JL
• NCBI Protein Information: ATP synthase subunit f, mitochondrial
• UniProt Protein Name: ATP synthase subunit f, mitochondrial
• UniProt Gene Name: ATP5J2
• UniProt Synonym Gene Names: ATP5JL
• UniProt Entry Name: ATPK_HUMAN

NCBI Description

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The catalytic portion of mitochondrial ATP synthase consists of five different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and single representatives of the gamma, delta, and epsilon subunits. The proton channel likely has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the f subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. This gene has multiple pseudogenes. Naturally occurring read-through transcription also exists between this gene and the downstream pentatricopeptide repeat domain 1 (PTCD1) gene. [provided by RefSeq, Nov 2010]