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Vascular Endothelial Growth Factor 111 Active Protein | flt1 active protein

Vascular Endothelial Growth Factor 111, Recombinant, Human (Vascular Endothelial Growth Factor A, VEGFA, VEGF-A, VEG, Vascular Permeability Factor, VPF)

Gene Names
flt1; flt1b; sFlt1; VEGFR1
Purity
Highly Purified
Purified 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1.0 EU/1ug (LAL).
Synonyms
Vascular Endothelial Growth Factor 111; Recombinant; Human (Vascular Endothelial Growth Factor A; VEGFA; VEGF-A; VEG; Vascular Permeability Factor; VPF); flt1 active protein
Ordering
For Research Use Only!
Purity/Purification
Highly Purified
Purified 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1.0 EU/1ug (LAL).
Form/Format
Supplied as a lyophilized powder from a sterile filtered solution in 30% CH3CN and 0.1% TFA.
Activity
Measured by its ability to stimulate proliferation of HUVE cells (Conn, G. et al., 1990, Proc. Natl. Acad. Sci. USA 87:1323-1327).
The ED50 for this effect is typically 0.6-3ng/ml.. The cell number is assessed in a fluorometric
assay using the redox sensitive dye, Resazurin.
Preparation and Storage
12 months from date of receipt, 20 to 70 degree C as supplied.
1 month, 2 to 8 degree C under sterile conditions after reconstitution.
3 months, 20 to 70 degree C under sterile conditions after reconstitution.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Related Product Information for flt1 active protein
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure (4). Humans normally express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206aa in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). Additionally, an active ~18kD form, VEGF111, can be induced in tumor cells by treatment with genotoxic agents and ultraviolet (UV-B) irradiation (5). Like VEGF121, VEGF111 lacks the basic heparin-binding region encoded by exons 6 and 7, and is freely diffusible (4, 5). VEGF111 also lacks major proteolytic cleavage sites encoded by exon 5, giving it additional stability. Human VEGF111 shares 87% aa sequence identity with corresponding regions of mouse and rat VEGF, 93% with feline, equine and bovine VEGF, and 91%, 95% and 96% with ovine, canine and porcine VEGF, respectively. All VEGF forms bind the single pass receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and in female during reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (6, 7).
Product Categories/Family for flt1 active protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
The methionyl form~13kD and a truncated form~12.8kD
The recombinant human VEGF 111 migrates as an ~12-13kD protein in
SDS-PAGE under reducing conditions.
NCBI Official Full Name
vascular endothelial growth factor receptor 1 isoform 1
NCBI Official Synonym Full Names
fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
NCBI Official Symbol
flt1
NCBI Official Synonym Symbols
flt1b; sFlt1; VEGFR1
NCBI Protein Information
vascular endothelial growth factor receptor 1; receptor tyrosine kinase Flt1b; soluble fms-like tyrosine kinase 1

Research Articles on flt1

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Product Notes

The flt1 (Catalog #AAA651044) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Vascular Endothelial Growth Factor 111, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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