VAP1 Active Protein | TAT1 active protein
VAP1, Recombinant, Human (Vascular Adhesion Protein 1, Membrane Primary Amine Oxidase, Copper Amine Oxidase, HPAO, Semicarbazide-sensitive Amine Oxidase, SSAO, VAP-1, AOC3)
Purity
Highly Purified~95% (SDS-PAGE, reducing conditions)
Synonyms
VAP1; Recombinant; Human (Vascular Adhesion Protein 1; Membrane Primary Amine Oxidase; Copper Amine Oxidase; HPAO; Semicarbazide-sensitive Amine Oxidase; SSAO; VAP-1; AOC3); TAT1 active protein
Purity/Purification
Highly Purified
~95% (SDS-PAGE, reducing conditions)
~95% (SDS-PAGE, reducing conditions)
Form/Format
Supplied as a liquid in HEPES, sodium chloride.
Application Notes
SDS-PAGE: 80-90kD, reducing conditions
Activity
Measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine.
Specific Activity
8 pmoles/min/ug.
Endotoxin Level
1EU/1ug (LAL)
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for TAT1 active protein
Vascular adhesion protein-1 (VAP1) is a copper amine oxidase with a topaquinone cofactor. VAP1 is a Type II integral membrane protein, but a soluble form of the enzyme is present in human serum, and its level increases in diabetes and some inflammatory liver diseases. VAP1 catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H2O2. The enzyme is sensitive to inhibition by semicarbazide. VAP1 expression is highest in the endothelium of lung, heart, and intestine, but low in tissues such as brain, spleen, kidney, and liver. VAP1 vascular expression is regulated at sites of inflammation through its release from intracellular granules in which the protein is stored. The adhesive function of VAP1 has been demonstrated in studies showing that the protein is important for the adherence of certain lymphocyte subtypes to inflamed endothelial tissues. VAP1 mediated adhesion is involved in the process of leukocyte extravasation, an important feature of inflammatory responses. The role of VAP1 amine oxidase activity in this process is not fully defined, but it appears to be carbohydrate-dependent. VAP1 is considered to be a therapeutic target for diabetes, oxidative stress, and inflammatory diseases.
Product Categories/Family for TAT1 active protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
85kD
NCBI Official Full Name
VAP1
UniProt Protein Name
Valine/tyrosine/tryptophan amino-acid permease 1
Protein Family
UniProt Gene Name
TAT1
UniProt Synonym Gene Names
TAP1; VAP1
UniProt Entry Name
TAT1_YEAST
Uniprot Description
Function: High-affinity transport of valine and tyrosine. Low-affinity transport of tryptophan. Can also transport L-cysteine. Ref.6
Subcellular location: Membrane; Multi-pass membrane protein.
Miscellaneous: Present with 504 molecules/cell in log phase SD medium.
Sequence similarities: Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family. [View classification]
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Product Notes
The VAP1 tat1 (Catalog #AAA635966) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. SDS-PAGE: 80-90kD, reducing conditions. Researchers should empirically determine the suitability of the VAP1 tat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VAP1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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