Rabbit SKA1 Polyclonal Antibody | anti-SKA1 antibody

Rabbit Polyclonal to Human SKA1

Cat. Num. AAA247783

• Gene Names: SKA1; C18orf24
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: SKA1; Polyclonal Antibody; Rabbit Polyclonal to Human SKA1; Anti-SKA1 Antibody (C-Terminus) IHC-plus; C18orf24; Human SKA1; anti-SKA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human SKA1
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 255

• Applicable Applications for anti-SKA1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (0.5 - 1 ug/ml); Usage: SKA1 antibody can be used for detection of SKA1 by Western blot at 0.5-1 ug/ml.
• Target Species: Human
• Immunogen Description: A 13 amino acid peptide from near the carboxy terminus of human SKA1.
• Immunogen Type: Synthetic peptide
• Immunogen: SKA1 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human SKA1.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-SKA1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SKA1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of SKA1 in A549 cell lysate with SKA1 antibody at (A) 0.5 and (B) 1 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 88014625
• NCBI GeneID: 220134
• NCBI Accession #: NP_001034624.1
• NCBI GenBank Nucleotide #: NM_001039535.2
• UniProt Accession #: Q96BD8; B2R9Y6; B4E0P4
• Molecular Weight: 24,390 Da
• NCBI Official Full Name: spindle and kinetochore-associated protein 1
• NCBI Official Synonym Full Names: spindle and kinetochore associated complex subunit 1
• NCBI Official Symbol: SKA1
• NCBI Official Synonym Symbols: C18orf24
• NCBI Protein Information: spindle and kinetochore-associated protein 1; spindle and KT (kinetochore) associated 1
• UniProt Protein Name: Spindle and kinetochore-associated protein 1
• Protein Family: Spindle and kinetochore-associated protein
• UniProt Gene Name: SKA1
• UniProt Synonym Gene Names: C18orf24
• UniProt Entry Name: SKA1_HUMAN

Uniprot Description

SKA1: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules. Belongs to the SKA1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: microtubule cytoskeleton; spindle microtubule; outer kinetochore of condensed chromosome; cytosol

Molecular Function: protein binding; microtubule binding

Biological Process: mitosis; cell division; mitotic cell cycle; regulation of microtubule polymerization or depolymerization; chromosome segregation