TNF ALPHA active protein

RECOMBINANT MOUSE TNF ALPHA

Cat. Num. AAA232328

• Gene Names: Tnf; DIF; Tnfa; TNF-a; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha
• Applications: ELISA, Functional Assay, Western Blot
• Purity: >98% by SDS PAGE and HPLC analysis
• Synonyms: TNF ALPHA; RECOMBINANT MOUSE TNF ALPHA; TNF ALPHA active protein

• Purity/Purification: >98% by SDS PAGE and HPLC analysis
• Form/Format: Purified protein produced in E Coli - lyophilised.
• Concentration: Approximate Protein Concentration: 0.1 mg/ml after reconstitution (varies by lot)
• Sequence Length: 219

• Applicable Applications for TNF ALPHA active protein: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Recombinant mouse TNFalpha may be used as the standard for ELISA applications with either anti-Mouse TNFalpha (purified) or anti-Mouse TNFalpha (biotin conjugate).; Western Blot: Recombinant mouse TNFalpha may be used as the positive control for western blotting applications with either anti-Mouse TNFalpha (purified) or anti-Mouse TNFalpha (biotin conjugate).; ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well; Functional Assays: Minimum Dilution: 0.05ng/ml; Maximum Dilution: 20ng/ml; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
• Reconstitution: Reconstitute with 0.2ml distilled water. For extended storage the addition of 0.1% BSA is recommended. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
• Buffer Solution: 10mM Sodium Phosphate
• Activity: 1 x 107 units/mg
• Target Species: Mouse
• Preparation and Storage: Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 3 months from date of reconstitution.

Testing Data #1

(Sandwich ELISA detection of murine TNFα using Rat anti Mouse TNFα (MBS212172) as a monoclonal capture antibody and biotinylated Rabbit anti Mouse TNFα (MBS220114) as a detection antibody with Streptavidin:HRP as the tertiary reagent for the detection of the antigen; recombinant Mouse TNFα (MBS232328) at the concentrations indicated on the x-axis)

Related Product Information for TNF ALPHA active protein

Tumour necrosis factor alpha (TNFalpha) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumour cells and certain other target cells.

NCBI and Uniprot Product Information

• NCBI GI #: 518831589
• NCBI GeneID: 21926
• NCBI Accession #: NP_001265530.1
• NCBI GenBank Nucleotide #: NM_001278601.1
• UniProt Accession #: P06804; O35853; Q62326; Q91VF3
• Molecular Weight: Protein Molecular Weight: 17.3kD (156 amino acid residues)
• NCBI Official Full Name: tumor necrosis factor isoform 2
• NCBI Official Synonym Full Names: tumor necrosis factor
• NCBI Official Symbol: Tnf
• NCBI Official Synonym Symbols: DIF; Tnfa; TNF-a; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha
• NCBI Protein Information: tumor necrosis factor; cachectin; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
• UniProt Protein Name: Tumor necrosis factor
• UniProt Gene Name: Tnf
• UniProt Synonym Gene Names: Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2
• UniProt Entry Name: TNFA_MOUSE

NCBI Description

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Apoptosis; Cytokine

Cellular Component: extracellular space; recycling endosome; cell surface; membrane; integral to plasma membrane; extracellular region; plasma membrane; integral to membrane; intracellular; external side of plasma membrane; secretory granule; lipid raft; phagocytic cup

Molecular Function: identical protein binding; protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; positive regulation of NFAT protein import into nucleus; activation of MAPK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; positive regulation of translational initiation by iron; positive regulation of membrane protein ectodomain proteolysis; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; tumor necrosis factor-mediated signaling pathway; cellular extravasation; positive regulation of phagocytosis; negative regulation of interleukin-6 production; JNK cascade; negative regulation of osteoblast differentiation; positive regulation of action potential; negative regulation of protein complex disassembly; positive regulation of cytokine production; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; response to virus; glucose metabolic process; positive regulation of interleukin-6 production; negative regulation of cytokine secretion during immune response; positive regulation of protein transport; DNA damage response, signal transduction resulting in induction of apoptosis; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; sequestering of triacylglycerol; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-18 production; signal transduction; chronic inflammatory response to antigenic stimulus; positive regulation of hair follicle development; negative regulation of cell proliferation; positive regulation of neuron apoptosis; positive regulation of cell proliferation; protein kinase B signaling cascade; positive regulation of chronic inflammatory response to antigenic stimulus; inflammatory response; regulation of protein amino acid phosphorylation; transformed cell apoptosis; positive regulation of peptidyl-serine phosphorylation; humoral immune response; regulation of cell proliferation; cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of interferon-gamma production; positive regulation of programmed cell death; positive regulation of protein complex assembly; negative regulation of viral genome replication; regulation of insulin secretion; positive regulation of JNK activity; positive regulation of osteoclast differentiation; multicellular organismal development; response to glucocorticoid stimulus; positive regulation of NF-kappaB import into nucleus; osteoclast differentiation; regulation of immunoglobulin secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 biosynthetic process; positive regulation of chemokine production; detection of mechanical stimulus involved in sensory perception of pain; cell activation; organ morphogenesis; defense response to Gram-positive bacterium; induction of apoptosis via death domain receptors; positive regulation of transcription factor activity; negative regulation of L-glutamate transport; negative regulation of transcription, DNA-dependent; leukocyte migration; apoptosis; positive regulation of smooth muscle cell proliferation; defense response; positive regulation of synaptic transmission; regulation of osteoclast differentiation; regulation of protein secretion; negative regulation of lipid catabolic process; lipopolysaccharide-mediated signaling pathway; regulation of I-kappaB kinase/NF-kappaB cascade; caspase activation; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of protein complex disassembly; MAPKKK cascade; calcium-mediated signaling; negative regulation of glucose import; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; positive regulation of protein kinase activity; positive regulation of fever; activation of MAPKKK activity; immune response; receptor biosynthetic process; positive regulation of protein amino acid phosphorylation; leukocyte tethering or rolling; negative regulation of myoblast differentiation; positive regulation of cytokine secretion; positive regulation of inflammatory response