Testing Data
(Recombinant Mouse TNF alpha (MBS232328) detected in a sandwich assay with Rat anti Mouse TNF alpha as the coating antibody; Rabbit anti Mouse TNF alpha:Biotin (MBS220114) as the detection antibody and Streptavidin:HRP (MBS238416) as the final layer)
Rat TNF ALPHA Monoclonal Antibody | anti-TNF ALPHA antibody
RAT ANTI MOUSE TNF ALPHA
Purified IgG - liquid
ELISA: Maximum Dilution: 5ug/ml
Flow Cytometry: Application Note: Membrane permeabilization is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
Preparation
Buffer Solution
Shelf Life: 18 months from date of despatch.
Testing Data
(Recombinant Mouse TNF alpha (MBS232328) detected in a sandwich assay with Rat anti Mouse TNF alpha as the coating antibody; Rabbit anti Mouse TNF alpha:Biotin (MBS220114) as the detection antibody and Streptavidin:HRP (MBS238416) as the final layer)
Testing Data
(Recombinant Mouse TNF alpha (MBS232328) detected in a sandwich assay with Rat anti Mouse TNF alpha as the coating antibody; Rabbit anti Mouse TNF alpha:Biotin (MBS220114) as the detection antibody and Streptavidin:HRP (MBS238416) as the final layer)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Uniprot Description
TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family.
Protein type: Apoptosis; Cytokine; Membrane protein, integral; Motility/polarity/chemotaxis
Cellular Component: extracellular space; cell surface; integral to plasma membrane; extracellular region; integral to membrane; lipid raft; secretory granule; recycling endosome; membrane; plasma membrane; intracellular; external side of plasma membrane; phagocytic cup
Molecular Function: identical protein binding; protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding
Biological Process: extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; positive regulation of NFAT protein import into nucleus; activation of MAPK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; positive regulation of translational initiation by iron; positive regulation of membrane protein ectodomain proteolysis; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; cellular extravasation; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; negative regulation of interleukin-6 production; JNK cascade; negative regulation of osteoblast differentiation; positive regulation of action potential; negative regulation of protein complex disassembly; positive regulation of cytokine production; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; response to virus; positive regulation of interleukin-6 production; glucose metabolic process; negative regulation of cytokine secretion during immune response; positive regulation of protein transport; DNA damage response, signal transduction resulting in induction of apoptosis; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; sequestering of triacylglycerol; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of interleukin-18 production; chronic inflammatory response to antigenic stimulus; positive regulation of hair follicle development; negative regulation of cell proliferation; positive regulation of neuron apoptosis; positive regulation of cell proliferation; protein kinase B signaling cascade; positive regulation of chronic inflammatory response to antigenic stimulus; inflammatory response; regulation of protein amino acid phosphorylation; transformed cell apoptosis; positive regulation of peptidyl-serine phosphorylation; humoral immune response; regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; cell proliferation; positive regulation of programmed cell death; positive regulation of interferon-gamma production; positive regulation of protein complex assembly; negative regulation of viral genome replication; regulation of insulin secretion; positive regulation of JNK activity; positive regulation of osteoclast differentiation; multicellular organismal development; response to glucocorticoid stimulus; positive regulation of NF-kappaB import into nucleus; osteoclast differentiation; regulation of immunoglobulin secretion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 biosynthetic process; positive regulation of chemokine production; cell activation; detection of mechanical stimulus involved in sensory perception of pain; defense response to Gram-positive bacterium; organ morphogenesis; induction of apoptosis via death domain receptors; positive regulation of transcription factor activity; negative regulation of L-glutamate transport; negative regulation of transcription, DNA-dependent; leukocyte migration; apoptosis; positive regulation of smooth muscle cell proliferation; defense response; positive regulation of synaptic transmission; regulation of osteoclast differentiation; regulation of protein secretion; negative regulation of lipid catabolic process; lipopolysaccharide-mediated signaling pathway; regulation of I-kappaB kinase/NF-kappaB cascade; caspase activation; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of protein complex disassembly; calcium-mediated signaling; MAPKKK cascade; negative regulation of glucose import; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; positive regulation of protein kinase activity; positive regulation of fever; activation of MAPKKK activity; immune response; receptor biosynthetic process; positive regulation of protein amino acid phosphorylation; negative regulation of myoblast differentiation; leukocyte tethering or rolling; positive regulation of inflammatory response; positive regulation of cytokine secretion
Research Articles on TNF ALPHA
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Product Notes
The TNF ALPHA tnf (Catalog #AAA212172) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TNF ALPHA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow cytometry (FC/FACS)*, Western Blot (WB). Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ELISA: Maximum Dilution: 5ug/ml Flow Cytometry: Application Note: Membrane permeabilization is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. . Researchers should empirically determine the suitability of the TNF ALPHA tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF ALPHA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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