Semaphorin 3A, Fc Chimera Active Protein
Semaphorin 3A, Fc Chimera, Recombinant, Mouse (Semaphorin III, Sema III, SEMA3A, SEMAD)
Purity
Purified90% by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1EU/ug (LAL)
Synonyms
Semaphorin 3A; Fc Chimera; Recombinant; Mouse (Semaphorin III; Sema III; SEMA3A; SEMAD); Fc Chimera active protein
Purity/Purification
Purified
90% by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1EU/ug (LAL)
90% by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1EU/ug (LAL)
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with PBS to 100ug/ml.
Biological Activity
Measured by its ability to cause collapse of chick dorsal root ganglia (DRG) neuron growth cones. The ED50 for this effect is typically 20-60ng/ml in the presence of 10ng/ml recombinant human beta-NGF.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Semaphorin 3A, Fc Chimera active protein
Semaphorin 3A is one of six Class 3 secreted semaphorins which share ~40-50% aa identity. Class 3 semaphorins are potent chemo- repellents that function in axon and/or vascular guidance during development. The 772aa mouse Sema3C contains a 20aa signal sequence, an ~500aa N-terminal Sema domain that forms a b-propeller structure similar to that found in integrin molecules, a PSI domain, a furin- type cleavage site, an Ig-like domain, and a C-terminal basic domain. Covalent dimerization plus cleavage at the C-terminus are required for activity of class 3 semaphorins. The 95kD mature mouse Sema3A shares at least 95% aa identity with human, rat, equine and canine Sema3A, and 90% and 86% aa identity with chick and zebrafish Sema3A, r espectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors. Sema3A signaling is transduced by plexin A1-4, indirectly via neuropilin-1. Sema3A activity is mediated by small GTPases that influence actin rearrangement and integrin activity. It is important in developmental organization of central and peripheral nerves, including those in heart, lung, kidneys, bones, teeth, and visual and olfactory systems. Gradients of Sema3A repel axons, but attract dendrites. Sema3A affect vasculogenesis by inhibiting integrin function and, with Sema3F, promoting apoptosis of endothelial cells. It is thought to suppress cancer-related angiogenesis. In the immune system, Sema3A influences T cell proliferation, migration, response to activation, and interactions with dendritic cells. It negatively regulates platelet activation. Expression of Sema3A in relevant parts of the nervous system may be increased in Alzheimer's disease, multiple sclerosis, ischemia and schizophrenia.
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Product Notes
The Semaphorin 3A, Fc Chimera (Catalog #AAA637196) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Semaphorin 3A, Fc Chimera, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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