RSPO-3 Active Protein | RSPO1 active protein

RSPO-3, Recombinant, Human (R-Spondin 3, Cristin-1) (BSA & Azide Free)

Cat. Num. AAA637004

• Gene Names: RSPO1; RSPO; CRISTIN3; RP11-566C13.1
• Purity: Purified; 95% (SDS­PAGE)
• Synonyms: RSPO-3; Recombinant; Human (R-Spondin 3; Cristin-1) (BSA & Azide Free); RSPO1 active protein

• Purity/Purification: Purified; 95% (SDS­PAGE)
• Form/Format: Supplied as a lyophilized powder from 0.2um filtered solution in PBS. Reconstitute with sterile 100ug/ml PBS.

• Application Notes: SDS-PAGE: 36-45kD, reducing conditions.
• Biological Activity: Measured by its ability to induce activation of b-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.5-2ng/ml
• Endotoxin Level: 1EU/ug (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for RSPO1 active protein

R-Spondin 3 (RSPO3, roof plate-specific spondin3), also called cysteine-rich and single thrombospondin domain containing-1 (Cristin 1), is a ~31kD secreted protein that shares ~40% amino acid identity with the other three R-Spondin family members (1, 2). All are positive modulators of Wnt /b-catenin signaling, but each has a distinct expression pattern (1-4). Like other R-spondins, R-Spondin3 contains two adjacent cysteine-rich furin-like domais (aa33-135) with one potential N-glycosylationsite (aa36), followed by a thrombospondin (TSP-1) motif (aa147-207) and a region rich in basic residues (aa211-269). Only the furin-like domains are needed for b-catenin stabilization (2). Within aa21-209, human R-Spondin 3 shares 93%, 92%, 97%, 96% and 92% aa identity with mouse, rat, equine, bovine and canine R-Spondin 3, respectively. Potential isoforms of 279 and 297 aa diverge at aa 210 and 276, respectively (5). Mouse R-Spondin 3 is critical for development of the placental labyrinthine layer, probably by promoting VEGF expression and thus vascular development (6, 7). It is also essential for expression of the placenta-specific transcription factor, Gcm1. In the mouse embryo, R-Spondin 3 is often expressed by or located near endothelial cells (6). It is found in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs (4, 7). R-Spondins regulate Wnt/b-catein by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1-mediated internalization (8, 9). Reports differ on whether R-Spondins bind LRP-6 directly (8-10). R-Spondin 3 has also been identified as an oncogene (11).

Product Categories/Family for RSPO1 active protein

Molecular Biology; MB-Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 339276103
• NCBI GeneID: 284654
• NCBI Accession #: NP_001229839.1
• NCBI GenBank Nucleotide #: NM_001242910.1
• UniProt Accession #: Q2MKA7; Q0H8S6; Q14C72; Q5T0F2; Q8N7L5; A2A420
• Molecular Weight: 28,959 Da
• NCBI Official Full Name: R-spondin-1 isoform 3
• NCBI Official Synonym Full Names: R-spondin 1
• NCBI Official Symbol: RSPO1
• NCBI Official Synonym Symbols: RSPO; CRISTIN3; RP11-566C13.1
• NCBI Protein Information: R-spondin-1; R-spondin homolog; roof plate-specific spondin-1
• UniProt Protein Name: R-spondin-1
• Protein Family: R-spondin
• UniProt Gene Name: RSPO1
• UniProt Synonym Gene Names: hRspo1
• UniProt Entry Name: RSPO1_HUMAN

NCBI Description

This gene is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. [provided by RefSeq, Jul 2008]

Uniprot Description

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: extracellular space; extracellular region; nucleus

Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; receptor binding

Biological Process: regulation of gene expression; regulation of receptor internalization; positive regulation of protein amino acid phosphorylation; Wnt receptor signaling pathway through beta-catenin; male meiosis; positive regulation of Wnt receptor signaling pathway

Disease: Palmoplantar Hyperkeratosis With Squamous Cell Carcinoma Of Skin And 46,xx Sex Reversal