RESISTIN Active Protein | RETN active protein

RECOMBINANT HUMAN RESISTIN

Cat. Num. AAA232425

• Gene Names: RETN; ADSF; RSTN; XCP1; FIZZ3; RETN1
• Applications: ELISA, Functional Assay, Western Blot
• Purity: >98% by SDS PAGE and HPLC analysis.
• Synonyms: RESISTIN; RECOMBINANT HUMAN RESISTIN; RETN active protein

• Purity/Purification: >98% by SDS PAGE and HPLC analysis.
• Form/Format: Purified recombinant protein - lyophilised
• Concentration: Total protein concentration 0.1mg/ml after reconstitution (varies by lot)
• Sequence Length: 108

• Applicable Applications for RETN active protein: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
• Reconstitution: Reconstitute with 250ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. ; Preparation
• Buffer Solution: 10mM Sodium Citrate
• Activity: Determined by its ability to stimulate lipolysis in cultured human adipocytes.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 3 months from date of reconstitution.

Related Product Information for RETN active protein

Human resistin, a disulfide-linked homodimeric protein otherwise known as adipose tissue-specific secretory factor, is an adipose-derived hormone and member of the resistin/FIZZ family, implicated as a suppressor of the ability of insulin to reduce blood sugar, and to play a role in type 2 diabetes and obesity. Studies have shown that resistin is involved in an increase in the transcription/expression of many pro-inflammatory cytokines, including interleukin-1,-6 and -12, and also upregulates vascular cell-adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1), and monocyte chemotactic protein-1 (MCP-1/CCL2).

NCBI and Uniprot Product Information

• NCBI GI #: 301129180
• NCBI GeneID: 56729
• NCBI Accession #: NP_001180303.1
• NCBI GenBank Nucleotide #: NM_001193374.1
• UniProt Accession #: Q9HD89; Q540D9; Q76B53; D6W649
• Molecular Weight: 19.5kD
• NCBI Official Full Name: resistin
• NCBI Official Synonym Full Names: resistin
• NCBI Official Symbol: RETN
• NCBI Official Synonym Symbols: ADSF; RSTN; XCP1; FIZZ3; RETN1
• NCBI Protein Information: resistin; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1; adipose tissue-specific secretory factor; c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; cysteine-rich secreted protein A12-alpha-like 2; cysteine-rich secreted protein FIZZ3; found in inflammatory zone 3; resistin delta2
• UniProt Protein Name: Resistin
• Protein Family: Resistin
• UniProt Gene Name: RETN
• UniProt Synonym Gene Names: FIZZ3; HXCP1; RSTN; ADSF
• UniProt Entry Name: RETN_HUMAN

NCBI Description

This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

resistin: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Belongs to the resistin/FIZZ family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular space; nucleus

Molecular Function: hormone activity

Biological Process: fat cell differentiation; response to mechanical stimulus; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell migration; positive regulation of synaptic transmission; response to insulin stimulus; aging

Disease: Diabetes Mellitus, Noninsulin-dependent