Progranulin Active Protein | granulin active protein
Progranulin, Recombinant, Human (Granulins, GRN, Proepithelin, PEPI)
Purity
Highly Purified~90% (HPLC, SDS-PAGE)
Synonyms
Progranulin; Recombinant; Human (Granulins; GRN; Proepithelin; PEPI); granulin active protein
Purity/Purification
Highly Purified
~90% (HPLC, SDS-PAGE)
~90% (HPLC, SDS-PAGE)
Form/Format
Supplied as a liquid in PBS.
Biological Activity
1.The effects of phospho-ERK1/2 and non phospho-ERK1/2 by human progranulin-DYKDDDDK in THP-1 cells. To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37 degree C 0, 30, 60, min, respectively by adding the recombinant protein (100ng/ml) to the THP-1 monocyte cell, which was maintained with serum starvation for 24hrs. Recombinant proteins in lanes 1, 2, and 3 were subjected to THP-1 monocyte cell treatments over 0, 30, 60 mins, respectively.
2. The effects of phospho-ERK1/2 and non phospho-ERK1/2 by human progranulin-DYKDDDDK in MCF10A cells. To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37 degree C over 0, 10, 30, 60 mins, respectively by adding the recombinant protein (500ng/ml) to the MCF10A human breast epithelial cells, which was maintained with serum starvation for 24hrs. Recombinant proteins in lanes 2, 3, and 4 were subjected to MCF10A human breast epithelial cell treatments over 0, 10, 30, 60 mins, respectively.
2. The effects of phospho-ERK1/2 and non phospho-ERK1/2 by human progranulin-DYKDDDDK in MCF10A cells. To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37 degree C over 0, 10, 30, 60 mins, respectively by adding the recombinant protein (500ng/ml) to the MCF10A human breast epithelial cells, which was maintained with serum starvation for 24hrs. Recombinant proteins in lanes 2, 3, and 4 were subjected to MCF10A human breast epithelial cell treatments over 0, 10, 30, 60 mins, respectively.
Endotoxin Level
<0.1ng/ug
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for granulin active protein
Progranulin, also called proepithelin and PC cell-derived growth factor, is a single precursor protein of granulins which are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. Granulins are a variety of active, 6kD peptides and named granulin A (epithelin 1), granulin B (epithelin 2), granulin C, etc. Both the peptides and intact progranulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Progranulin Human Recombinant fused to DYKDDDDK at C-terminus produced in HEK is a single, glycosylated, polypeptide chain containing 593aa and having a molecular mass of 88kD. The Progranulin is purified by standard chromatographic techniques.
Product Categories/Family for granulin active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~88kD
NCBI Official Full Name
progranulin
NCBI Official Symbol
granulin
NCBI Protein Information
progranulin
Similar Products
Product Notes
The granulin (Catalog #AAA635628) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Progranulin, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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