Chromatin Immunoprecipitation (ChIP)
(PARP-2 antibody tested by ChIP analysis. Chromatin IP performed on stably transfected AC16 cells using the per-immune serum (Pre) or the PARP-2 antibody. ChIP DNA was subjected to qPCR using primers specific for human CD36 gene. Data are presented relative to input.)
Rabbit anti-Human PARP-2 Polyclonal Antibody | anti-PARP-2 antibody
PARP-2 antibody (pAb)
ChIP: 2-10ul per ChIP
IP: 2-10ul per IP
WB: 1:500 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
Chromatin Immunoprecipitation (ChIP)
(PARP-2 antibody tested by ChIP analysis. Chromatin IP performed on stably transfected AC16 cells using the per-immune serum (Pre) or the PARP-2 antibody. ChIP DNA was subjected to qPCR using primers specific for human CD36 gene. Data are presented relative to input.)
Chromatin Immunoprecipitation (ChIP)
(PARP-2 antibody tested by ChIP analysis. Chromatin IP performed on stably transfected AC16 cells using the per-immune serum (Pre) or the PARP-2 antibody. ChIP DNA was subjected to qPCR using primers specific for human CD36 gene. Data are presented relative to input.)
Western Blot (WB)
(PARP-2 antibody used for immunoprecipitation. Western blot of immunoprecipitation using the PARP-2 antibody. Lane 1: input (AC16 whole cell lystate). Lane 2: IP using pre-immune serum. Lane 3: IP using PARP-2 antibody.)
Western Blot (WB)
(PARP-2 antibody used for immunoprecipitation. Western blot of immunoprecipitation using the PARP-2 antibody. Lane 1: input (AC16 whole cell lystate). Lane 2: IP using pre-immune serum. Lane 3: IP using PARP-2 antibody.)
Western Blot (WB)
(PARP-2 antibody tested by Western blot. HeLa cell nuclear extract (20 ug) was probed with PARP-2 antibody at a dilution of 1:1000.)
Western Blot (WB)
(PARP-2 antibody tested by Western blot. HeLa cell nuclear extract (20 ug) was probed with PARP-2 antibody at a dilution of 1:1000.)
Background: PARP-2 encodes a chromatin-associated enzyme, a poly-ADP ribosyl transferase, which modifies various nuclear proteins by poly-ADP ribosylation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. PARP-2 is cleaved by caspase enzymes during the process of apoptosis.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jul 2008]
Uniprot Description
PARP2: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA repair, damage; Transferase; EC 2.4.2.30
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: protein binding; DNA binding; NAD+ ADP-ribosyltransferase activity
Biological Process: protein amino acid ADP-ribosylation; base-excision repair; DNA repair
Research Articles on PARP-2
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Product Notes
The PARP-2 parp2 (Catalog #AAA388339) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PARP-2 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARP-2 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB). Applications Validated: ChIP: 2-10ul per ChIP IP: 2-10ul per IP WB: 1:500 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Researchers should empirically determine the suitability of the PARP-2 parp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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