Plexin C1 Active Protein | PLXNC1 active protein

Plexin C1, Recombinant, Mouse (Plexin-C1, Virus-encoded Semaphorin Protein Receptor, CD232, PLXNC1, VESPR)

Cat. Num. AAA635720

• Gene Names: PLXNC1; CD232; VESPR; PLXN-C1
• Purity: Highly Purified; ~95% (SDS-PAGE, reducing conditions)
• Synonyms: Plexin C1; Recombinant; Mouse (Plexin-C1; Virus-encoded Semaphorin Protein Receptor; CD232; PLXNC1; VESPR); PLXNC1 active protein

• Purity/Purification: Highly Purified; ~95% (SDS-PAGE, reducing conditions)
• Form/Format: Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS.

• Application Notes: SDS-PAGE: 160-170kD, reducing conditions
• Activity: Measured by its binding ability in a functional ELISA. When recombinant mouse Plexin C1 is coated at 5ug/ml (100ul/well), recombinant mouse Semaphorin 7A/Fc Chimera binds with an apparent KD 5nM.
• Endotoxin Level: 1EU/1ug (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for PLXNC1 active protein

Plexin C1, also known as Virus-encoded semaphorin protein receptor (VESPR) and CD232, is a 210kD type I transmembrane glycoprotein in the C subfamily of the plexin family. Human Plexin C1 contains a 34aa signal sequence, a 910aa extracellular domain (ECD) with one Sema­domain and two cysteine­rich Met­related sequences (MRS), a 21aa transmembrane domain and a 603aa cytoplasmic domain that includes features common to other plexins, including a phosphothreonine site. The mouse Plexin C1 ECD shares 85%, 78% and 65% aa identity with human, bovine and equine Plexin C1, respectively. A truncated rat Plexin C1 cDNA that encodes an apparently soluble form shares 93% aa identity with mouse Plexin C1 within aa 263­932. Plexin C1 is widely expressed in neuronal and non­neuronal fetal and adult tissues. In neuronal development, its role is unclear. Semaphorin­7a (Sema7a, CD108) binds Plexin C1 in vitro and the two show a similar expression pattern during rat neuronal development, but beta-1 integrins rather than Plexin C1 appear to mediate Sema7a effects on axon outgrowth. Plexin C1 does appear to play a role in partitioning of paraventricular and supraoptic neurons in the hypothalamus, as indicated by specific defects seen in mice deleted for Plexin C1 In the immune system, effects of Sema7a on T cell­mediated inflammatory responses also appear to be mediated by beta-1 integrins rather than plexins. However, Plexin C1 may function to oppose the effect of beta-1 integrins, as it does on Sema7a- mediated spreading and dendrite formation in melanocytes Its expression is lost in melanoma metastasis, suggesting a role as a tumor suppressor Plexin C1 is the receptor for poxvirus (A39R protein) and herpes virus (AHVsema) semaphorin homologs, and mediates activation of monocytes and inhibition of dendritic cell and neutrophil phagocytosis and migration by A39R.

Product Categories/Family for PLXNC1 active protein

Molecular Biology; MB-Plexin; Semaphorin

NCBI and Uniprot Product Information

• NCBI GI #: 5032223
• NCBI GeneID: 10154
• NCBI Accession #: NP_005752.1
• NCBI GenBank Nucleotide #: NM_005761.2
• UniProt Accession #: O60486; Q59H25
• Molecular Weight: 101.6kD
• NCBI Official Full Name: plexin-C1
• NCBI Official Synonym Full Names: plexin C1
• NCBI Official Symbol: PLXNC1
• NCBI Official Synonym Symbols: CD232; VESPR; PLXN-C1
• NCBI Protein Information: plexin-C1; plexin (semaphorin receptor); receptor for viral semaphorin protein; receptor for virally-encoded semaphorin; virus-encoded semaphorin protein receptor
• UniProt Protein Name: Plexin-C1
• Protein Family: Plexin
• UniProt Gene Name: PLXNC1
• UniProt Synonym Gene Names: VESPR
• UniProt Entry Name: PLXC1_HUMAN

NCBI Description

This gene encodes a member of the plexin family. Plexins are transmembrane receptors for semaphorins, a large family of proteins that regulate axon guidance, cell motility and migration, and the immune response. The encoded protein and its ligand regulate melanocyte adhesion, and viral semaphorins may modulate the immune response by binding to this receptor. The encoded protein may be a tumor suppressor protein for melanoma. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

PLXNC1: Receptor for SEMA7A, for smallpox semaphorin A39R, vaccinia virus semaphorin A39R and for herpesvirus Sema protein. Binding of semaphorins triggers cellular responses leading to the rearrangement of the cytoskeleton and to secretion of IL6 and IL8. Belongs to the plexin family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 12q23.3

Cellular Component: membrane; integral to membrane; plasma membrane; intracellular

Molecular Function: protein binding; receptor activity; receptor binding

Biological Process: axon guidance; cell adhesion; signal transduction